Results 241 to 250 of about 1,033,178 (294)
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Rapid transformation of Medicago truncatula : regeneration via shoot organogenesis
Plant Cell Reports, 1996A rapid transformation and regeneration system has been developed forM. truncatula cv Jemalong (barrel medic) by which it is possible to obtain transgenic plants within 2.5 months. The procedure involvesAgrobacterium-mediated transformation of cotyledon explants coupled with the regeneration of transformed plants via direct organogenesis.
A T, Trieu, M J, Harrison
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Regeneration and micrografting of lentil shoots
In Vitro Cellular & Developmental Biology - Plant, 2001A protocol for regeneration and micrografting of shoots of lentil (Lens culinaris Medik) was developed. Multiple shoots (4–5) were regenerated from cotyledonary node explants on Murashige and Skoog (MS) medium containing 8.8 μM 6-benzylaminopurine. In vitro regenerated shoots were micrografted on rootstocks with 96% efficiency.
Anju Gulati, Pat Schryer, Alan McHughen
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The molecular path to in vitro shoot regeneration
Biotechnology Advances, 2014Plant regeneration through de novo shoot organogenesis in tissue culture is a critical step in most plant transformation and micropropagation procedures. Establishing an efficient regeneration protocol is an empirical process and requires optimization of multiple factors that influence the regeneration capacity. Here, we review the molecular process of
Hans, Motte +3 more
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Transformed callus does not necessarily regenerate transformed shoots
Plant Cell Reports, 1988Agrobacterium tumefaciens carrying a disarmed Ti plasmid vector was used for the transformation of flax tissue. Transformed callus was obtained from inoculated hypocotyl segments and healthy green shoots were regenerated from this callus. Nopaline assays on shoot tissue were positive for nopaline content if carried out soon after removal of the shoot ...
M C, Jordan, A, McHughen
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In vitro plant regeneration from callus of shoot apices in apple shoot culture
Plant Cell Reports, 2000A new reliable protocol for the induction of adventitious shoot formation and plant regeneration from apple callus has been developed. High regeneration frequency was obtained with this method in four different genotypes (Jork9, M26, Gala and McIntosh) and callus maintained regeneration ability for several months.
E. Caboni, P. Lauri, D'ANGELI, Simone
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Plant Cell Reports, 2007
We have investigated the role of ethylene in shoot regeneration from cotyledon explants of Arabidopsis thaliana. We examined the ethylene sensitivity of five ecotypes representing both poor and prolific shoot regenerators and identified Dijon-G, a poor regenerator, as an ecotype with dramatically enhanced ethylene sensitivity.
Steven P, Chatfield, Manish N, Raizada
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We have investigated the role of ethylene in shoot regeneration from cotyledon explants of Arabidopsis thaliana. We examined the ethylene sensitivity of five ecotypes representing both poor and prolific shoot regenerators and identified Dijon-G, a poor regenerator, as an ecotype with dramatically enhanced ethylene sensitivity.
Steven P, Chatfield, Manish N, Raizada
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Improvements in rooting regenerated safflower (Carthamus tinctorius L.) shoots
Plant Cell Reports, 1996A continuing obstacle for regenerating safflower (Carthamus tinctorius L.) plants from cultured explants or callus has been a reliable method for rooting shoots. For shoots directly regenerated from primary explants, 76% of shoots rooted after a 7-d exposure to 10 mg/1 indole-3-butyric acid.
C M, Baker, W E, Dyer
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Plant and Cell Physiology, 2019
Plant cells have a remarkable plasticity that allows cellular reprogramming from differentiated cells and subsequent tissue regeneration. Callus formation occurs from pericycle-like cells through a lateral root developmental pathway, and even aerial ...
Kyounghee Lee +3 more
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Plant cells have a remarkable plasticity that allows cellular reprogramming from differentiated cells and subsequent tissue regeneration. Callus formation occurs from pericycle-like cells through a lateral root developmental pathway, and even aerial ...
Kyounghee Lee +3 more
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Shoot regeneration and micrografting of micropropagated hybridtomatoes
The Journal of Horticultural Science and Biotechnology, 2005SummaryThe use of grafted tomato plants is expanding. An in vitro propagation system was established for three commercial F1 cultivars (‘Garnet 622’, ‘Jumbo’ and ‘Marvel’) to combine micropropagation with micrografting. Nodal tissue explants were excised from the central stem of plantlets and micropropagated on MS medium.
I. Grigoriadis +2 more
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Shoot regeneration from protoplasts of Ulmus × ‘Pioneer’
Plant Science, 1986Abstract Protoplasts were isolated from leaf-derived calli of Ulmus × ‘Pioneer’ with a yield of 0.2 × 10 3 –2.0 × 10 5 protoplasts/gram of callus. Protoplasts were cultured in KM medium 8p supplemented with 1.0 μM 2,4-Dichlorophenoxyacetic acid (2,4-D), 2.5 μM naphthaleneacetic acid (NAA), 1 μM zeatin, 0.38 M glucose and 0.7 mM ...
Masomeh Behrouz Sticklen +2 more
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