Results 111 to 120 of about 5,590,230 (287)
Utility analyses of AVITI sequencing chemistry
BMC GenomicsBackground DNA sequencing is a critical tool in modern biology. Over the last two decades, it has been revolutionized by the advent of massively parallel sequencing, leading to significant advances in the genome and transcriptome sequencing of various ...
Silvia Liu +12 more
doaj +1 more source
USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival
Molecular Oncology, EarlyView.CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna +8 more
wiley +1 more source
Efficient analysis solution for DNA short-read sequencing
, 2017 In recent years, the demand for DNA sequencing analysis has been boosted with the advance of DNA sequencing technologies; exceeding the capacities of high-end computer servers. This thesis presents integrated software solutions for popular DNA Sequencing analyses, along with implementation and experiments with real data to demonstrate the strength of ...
openaire +2 more sources
Molecular Oncology, EarlyView.Enterotoxigenic Bacteroides fragilis is a gut bacterium linked to bowel cancer. We propose that its toxin acts along two routes: at the cell surface, cutting the adhesion protein E‐cadherin and triggering inflammation; and inside the cell, delivered by bacterial vesicles, switching on NOD1 and cancer stem cell programs.
Kangchan Choi +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Whole-genome sequencing and assembly with high-throughput, short-read technologies.
PLoS ONE, 2007 While recently developed short-read sequencing technologies may dramatically reduce the sequencing cost and eventually achieve the $1000 goal for re-sequencing, their limitations prevent the de novo sequencing of eukaryotic genomes with the standard ...
Andreas Sundquist +4 more
doaj +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
The novel allele HLA‐A*02:04:03 was detected with long‐read sequencing but failed to be detected by short‐read sequencing.
Pernille Koefoed‐Nielsen +1 more
core +1 more source
K-MARVEL: K-Mer-based antimicrobial resistance virtual exploration lab
Nature CommunicationsThe rapid spread of antimicrobial resistance (AMR) necessitates new computational surveillance tools. Current methods for analysis of next-generation sequencing data have trade-offs: assembly-based approaches are computationally intensive, and direct ...
Nirmal Singh Mahar +4 more
doaj +1 more source
Combining panel-based and whole-transcriptome-based gene fusion detection by long-read sequencing
Cell Reports: MethodsSummary: We present a comprehensive gene fusion (GF) detection and analysis workflow that combines targeted panel-based and whole-transcriptome long-read sequencing.
Karleena Rybacki +8 more
doaj +1 more source