Results 101 to 110 of about 5,590,230 (287)

Reprogramming tumor cells with LCOR mRNA nanotherapy to enhance immunotherapy

Molecular Oncology, EarlyView.
LCOR (Ligand‐dependent corepressor) mRNA encapsulated in polymeric nanoparticles restores LCOR activity in tumor cells, constraining aggressive tumor cell states and increasing tumor immunogenicity. This reprogramming makes tumor cells more vulnerable to T‐cell attack and enhances their response to immune‐checkpoint immunotherapy.
Gabriel Serra‐Mir, Elena Haro‐Martínez, Sandra Blasco‐Benito, Chiara Cannatà, José Ángel Palomeque, Anna López‐Plana, Maria Sanz‐Flores, Marc Sabartés, Cristina Fornaguera, Toni Celià‐Terrassa   +9 more
wiley   +1 more source

Genome-resolved metagenomics using short-, long-read and metaHiC sequencing

, 2022
Reference-quality metagenome-assembled genomes (MAGs) are the key to exploring microbial compositions and microbe-phenotype associations. They can be recovered by different sequencing technologies and computational tools, which need an unbiased and ...
Longhao Jia
core   +1 more source

Comparative metagenomic assessment of Illumina-compatible library preparation methods, short-read lengths, and PacBio HiFi sequencing reveals differences in microbial and functional diversity recovery from a complex environmental sample

Microbiology Spectrum
Metagenomics enables comprehensive exploration of microbial communities but is influenced by library preparation and sequencing technologies, affecting recovery of microbial genomes and proteins.
Rubén Díaz-Rúa, Daniela I. Drautz-Moses, Xiang Zhao, Sadhasivam Perumal, Luke Esau, Angel Angelov, Alexander Putra, Patrick Driguez, Ming Sin Cheung, Emanuele Palescandolo   +9 more
doaj   +1 more source

Resolving the Complexity of Human Skin Metagenomes Using Single-Molecule Sequencing

mBio, 2016
Deep metagenomic shotgun sequencing has emerged as a powerful tool to interrogate composition and function of complex microbial communities. Computational approaches to assemble genome fragments have been demonstrated to be an effective tool for de novo ...
Yu-Chih Tsai, Sean Conlan, Clayton Deming, Julia A. Segre, Heidi H. Kong, Jonas Korlach, Julia Oh   +6 more
doaj   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Short-read and long-read RNA sequencing of mouse hematopoietic stem cells at bulk and single-cell levels

, 2021
Hematopoietic stem cells (HSCs) lie at the top of the differentiation hierarchy. Although HSC and their immediate downstream, multipotent progenitors (MPP) have full multilineage differentiation capacity, only long-term (LT-) HSC have the capacity for ...
Xiuran Zheng (10870052)
core   +1 more source

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

Short-Read Sequencing Benchmarking with Donor-Specific Assemblies

Abstract Background High-throughput short-read sequencing has become a core technology for genomics, but the rapid expansion of available platforms has made it increasingly important to benchmark them under standardized conditions.
Sean R. McGee, Joshua D. Smith, Christian D. Frazar, Erica Ryke, Mitchell R. Vollger, Youngjun Kwon, James T. Bennett, Evan E. Eichler, Andrew B. Stergachis, Chia-Lin Wei   +9 more
openaire   +2 more sources

An algorithm for mapping short reads to a dynamically changing genomic sequence

Journal of Discrete Algorithms, 2012
AbstractNext-generation sequencing technologies have redefined the way genome sequencing is performed. They are able to produce tens of millions of short sequences (reads), during a single experiment, and with a much lower cost than previously possible. Due to the dramatic increase in the amount of data generated, a challenging task is to map (align) a
Costas S. Iliopoulos, Derrick G. Kourie, Laurent Mouchard, Themba K. Musombuka, Solon P. Pissis, Corné de Ridder   +5 more
openaire   +3 more sources

A Cloud Infrastructure for Optimization of a Massive Parallel Sequencing Workflow [PDF]

, 2012
Massive Parallel Sequencing is a term used to describe several revolutionary approaches to DNA sequencing, the so-called Next Generation Sequencing technologies.
Rosalba Provenzano, Provenzano, R., Andrea Acquaviva, Olivier Terzo, Provenzano R., Lorenzo Mossucca, Terzo, Olivier, Mossucca, L., Acquaviva, Andrea, Mossucca L, Abate, Francesco, Francesco Abate   +11 more
core   +1 more source