Results 1 to 10 of about 7,946,195 (290)

Long-read sequencing for brain tumors [PDF]

Frontiers in Oncology
Brain tumors and genomics have a long-standing history given that glioblastoma was the first cancer studied by the cancer genome atlas. The numerous and continuous advances through the decades in sequencing technologies have aided in the advanced ...
William J. Shelton, Sara Zandpazandi, J Stephen Nix, Murat Gokden, Michael Bauer, Katie Rose Ryan, Christopher P. Wardell, Olena Morozova Vaske, Analiz Rodriguez   +8 more
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Long-Read Sequencing Emerging in Medical Genetics [PDF]

Frontiers in Genetics, 2019
The wide implementation of next-generation sequencing (NGS) technologies has revolutionized the field of medical genetics. However, the short read lengths of currently used sequencing approaches pose a limitation for the identification of structural ...
Tuomo Mantere, Tuomo Mantere, Simone Kersten, Simone Kersten, Simone Kersten, Alexander Hoischen, Alexander Hoischen, Alexander Hoischen   +7 more
doaj   +7 more sources

Why Are Long-Read Sequencing Methods Revolutionizing Microbiome Analysis?

Microorganisms
Most of the knowledge available on the composition and functionality of microbial communities in different ecosystems comes from short-read sequencing methods.
Adriana González, Asier Fullaondo, Adrian Odriozola   +2 more
doaj   +4 more sources

Application of long-read sequencing to the detection of structural variants in human cancer genomes

Computational and Structural Biotechnology Journal, 2021
In recent years, the so-called long-read sequencing technology has had a substantial impact on various aspects of genome sciences. Here, we introduce recent studies of cancerous structural variants (SVs) using long-read sequencing technologies, namely ...
Yoshitaka Sakamoto, Suzuko Zaha, Yutaka Suzuki, Masahide Seki, Ayako Suzuki   +4 more
doaj   +3 more sources

Long-read sequencing transcriptome quantification with lr-kallisto. [PDF]

PLoS Computational Biology
RNA abundance quantification has become routine and affordable thanks to high-throughput "short-read" technologies that provide accurate molecule counts at the gene level.
Rebekah K Loving, Delaney K Sullivan, Fairlie Reese, Elisabeth Rebboah, Jasmine Sakr, Narges Rezaie, Heidi Y Liang, Ghassan Filimban, Shimako Kawauchi, A Sina Booeshaghi, Páll Melsted, Conrad Oakes, Diane Trout, Brian A Williams, Grant R MacGregor, Barbara J Wold, Ali Mortazavi, Lior Pachter   +17 more
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DNA read count calibration for single-molecule, long-read sequencing

Scientific Reports, 2022
There are many applications in which quantitative information about DNA mixtures with different molecular lengths is important. Gene therapy vectors are much longer than can be sequenced individually via short-read NGS.
Luis M. M. Soares, Terrence Hanscom, Donald E. Selby, Samuel Adjei, Wei Wang, Dariusz Przybylski, John F. Thompson   +6 more
doaj   +1 more source

Long-read sequencing in fungal identification [PDF]

Microbiology Australia, 2022
Long-read sequencing is currently supported by sequencing platforms from Pacific Biosciences and Oxford Nanopore Technologies, both of which generate ultra-long reads.
Wieland Meyer, Laszlo Irinyi, Minh Thuy Vi Hoang   +2 more
doaj  

Are we there yet? Benchmarking low-coverage nanopore long-read sequencing for the assembling of mitochondrial genomes using the vulnerable silky shark Carcharhinus falciformis

BMC Genomics, 2022
Background Whole mitochondrial genomes are quickly becoming markers of choice for the exploration of within-species genealogical and among-species phylogenetic relationships. Most often, ‘primer walking’ or ‘long PCR’ strategies plus Sanger sequencing or
J. Antonio Baeza, F. J. García-De León
doaj   +1 more source

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

African Journal of Laboratory Medicine, 2020
No abstract available.
Boluwatife A. Adewale
doaj   +1 more source

Ultra-accurate microbial amplicon sequencing with synthetic long reads

Microbiome, 2021
Background Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing.
Benjamin J. Callahan, Dmitry Grinevich, Siddhartha Thakur, Michael A. Balamotis, Tuval Ben Yehezkel   +4 more
doaj   +1 more source