Results 1 to 10 of about 680,559 (291)

Long-read sequencing transcriptome quantification with lr-kallisto. [PDF]

PLoS Computational Biology
RNA abundance quantification has become routine and affordable thanks to high-throughput "short-read" technologies that provide accurate molecule counts at the gene level.
Rebekah K Loving, Delaney K Sullivan, Fairlie Reese, Elisabeth Rebboah, Jasmine Sakr, Narges Rezaie, Heidi Y Liang, Ghassan Filimban, Shimako Kawauchi, A Sina Booeshaghi, Páll Melsted, Conrad Oakes, Diane Trout, Brian A Williams, Grant R MacGregor, Barbara J Wold, Ali Mortazavi, Lior Pachter   +17 more
doaj   +4 more sources

Long-read sequencing for diagnosis of genetic myopathies [PDF]

BMJ Neurology Open
Genetic myopathies are caused by pathogenic variants in >300 genes across the nuclear and mitochondrial genomes. Although short-read next-generation sequencing (NGS) has revolutionised the diagnosis of genetic disorders, large and/or complex genetic ...
Gianina Ravenscroft, Roula Ghaoui, Ryan Davis, Kishore Raj Kumar, Karl Ng, Marina Kennerson, Dennis Yeow, Ira Deveson, Laura Ivete Rudaks, Pak Leng Cheong   +9 more
doaj   +4 more sources

Long-Read Sequencing Emerging in Medical Genetics [PDF]

Frontiers in Genetics, 2019
The wide implementation of next-generation sequencing (NGS) technologies has revolutionized the field of medical genetics. However, the short read lengths of currently used sequencing approaches pose a limitation for the identification of structural ...
Tuomo Mantere, Tuomo Mantere, Simone Kersten, Simone Kersten, Simone Kersten, Alexander Hoischen, Alexander Hoischen, Alexander Hoischen   +7 more
doaj   +5 more sources

Long-read sequencing for brain tumors [PDF]

Frontiers in Oncology
Brain tumors and genomics have a long-standing history given that glioblastoma was the first cancer studied by the cancer genome atlas. The numerous and continuous advances through the decades in sequencing technologies have aided in the advanced ...
William J. Shelton, Sara Zandpazandi, J Stephen Nix, Murat Gokden, Michael Bauer, Katie Rose Ryan, Christopher P. Wardell, Olena Morozova Vaske, Analiz Rodriguez   +8 more
doaj   +4 more sources

Long-read sequencing in fungal identification [PDF]

Microbiology Australia, 2022
Long-read sequencing is currently supported by sequencing platforms from Pacific Biosciences and Oxford Nanopore Technologies, both of which generate ultra-long reads.
Wieland Meyer, Laszlo Irinyi, Minh Thuy Vi Hoang   +2 more
doaj   +2 more sources

Why Are Long-Read Sequencing Methods Revolutionizing Microbiome Analysis? [PDF]

Microorganisms
Most of the knowledge available on the composition and functionality of microbial communities in different ecosystems comes from short-read sequencing methods.
Adriana González, Asier Fullaondo, Adrian Odriozola   +2 more
doaj   +2 more sources

Long-Read Nanopore-Based Sequencing of Anelloviruses

Viruses
Routinely used metagenomic next-generation sequencing (mNGS) techniques often fail to detect low-level viremia (
Raghavendran Anantharam, Dylan Duchen, Andrea L. Cox, Winston Timp, David L. Thomas, Steven J. Clipman, Abraham J. Kandathil   +6 more
doaj   +3 more sources

Improving Bacterial Metagenomic Research through Long-Read Sequencing

Microorganisms, 2023
Metagenomic sequencing analysis is central to investigating microbial communities in clinical and environmental studies. Short-read sequencing remains the primary approach for metagenomic research; however, long-read sequencing may offer advantages of ...
Noah Greenman, Sayf Al-Deen Hassouneh, Latifa S. Abdelli, Catherine Johnston, Taj Azarian   +4 more
doaj   +3 more sources

Genome sequencing using long-read sequencing

Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 2023
Juan Guillermo McEwen, Oscar Mauricio Gómez   +1 more
doaj   +2 more sources

Nanopore long-read sequencing of circRNAs [PDF]

Methods, 2021
Circular RNA (circRNA) is a group of highly stable RNA molecules with suggested roles in development and disease. They derive from linear pre-mRNAs when a 5'-splice site splices back to an upstream 3'-splice site in a process termed back-splicing. Most circRNAs are multi-exonic and may contain several thousand nucleotides.
Rahimi, Karim, Færch Nielsen, Anne, Venø, Morten T., Kjems, Jørgen   +3 more
openaire   +2 more sources