Results 31 to 40 of about 8,328,477 (294)

Are we there yet? Benchmarking low-coverage nanopore long-read sequencing for the assembling of mitochondrial genomes using the vulnerable silky shark Carcharhinus falciformis

BMC Genomics, 2022
Background Whole mitochondrial genomes are quickly becoming markers of choice for the exploration of within-species genealogical and among-species phylogenetic relationships. Most often, ‘primer walking’ or ‘long PCR’ strategies plus Sanger sequencing or
J. Antonio Baeza, F. J. García-De León
doaj   +1 more source

Long-read sequencing report with Oxford Nanopore Technologies of different soil samples using different DNA extraction methods.

, 2023
Long-read sequencing report with Oxford Nanopore Technologies of different soil samples using different DNA extraction ...
Andrey Zamyatnin (16857811), Olga Samuilova (16857733), Ulykbek Kairov (16857807), Konstantin I. Ivanov (16857730), Ruslan Kalendar (16857663)   +4 more
core   +1 more source

HAT: de novo variant calling for highly accurate short-read and long-read sequencing data [PDF]

, 2023
MOTIVATION: de novo variant (DNV) calling is challenging from parent-child sequenced trio data. We developed H are A nd T ortoise (HAT) to work as an automated workflow to detect DNVs in highly accurate short-read and long-read sequencing data.
Ng, Jeffrey K., Turner, Tychele N., Ng, Jeffrey K, Turner, Tychele N, Jeffrey K. Ng, Tychele N. Turner   +5 more
core   +1 more source

Long-read sequencing report with Oxford Nanopore Technologies of different soil samples using different DNA extraction methods (Report 20.02.2023)

, 2023
Long-read sequencing report with Oxford Nanopore Technologies of different soil samples using different DNA extraction methods.
Andrey Zamyatnin (16857811), Olga Samuilova (16857733), Ulykbek Kairov (16857807), Konstantin I. Ivanov (16857730), Ruslan Kalendar (16857663)   +4 more
core   +1 more source

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

African Journal of Laboratory Medicine, 2020
No abstract available.
Boluwatife A. Adewale
doaj   +1 more source

Ultra-accurate microbial amplicon sequencing with synthetic long reads

Microbiome, 2021
Background Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing.
Benjamin J. Callahan, Dmitry Grinevich, Siddhartha Thakur, Michael A. Balamotis, Tuval Ben Yehezkel   +4 more
doaj   +1 more source

Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies

Microbiome, 2023
Background Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches.
Luis H. Orellana, Karen Krüger, Chandni Sidhu, Rudolf Amann   +3 more
doaj   +1 more source

Long-read sequencing settings for efficient structural variation detection based on comprehensive evaluation

BMC Bioinformatics, 2021
Background With the rapid development of long-read sequencing technologies, it is possible to reveal the full spectrum of genetic structural variation (SV).
Tao Jiang, Shiqi Liu, Shuqi Cao, Yadong Liu, Zhe Cui, Yadong Wang, Hongzhe Guo   +6 more
doaj   +1 more source

Genome Sequencing and Assembly by Long Reads in Plants [PDF]

Genes, 2017
Plant genomes generated by Sanger and Next Generation Sequencing (NGS) have provided insight into species diversity and evolution. However, Sanger sequencing is limited in its applications due to high cost, labor intensity, and low throughput, while NGS reads are too short to resolve abundant repeats and polyploidy, leading to incomplete or ambiguous ...
Changsheng Li, Feng Lin, Dong An, Wenqin Wang, Ruidong Huang   +4 more
openaire   +2 more sources

Long-read sequence assembly: a technical evaluation in barley [PDF]

The Plant Cell, 2021
AbstractSequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial computational resources and often several complementary sequence assembly and genome mapping approaches. The recent development of fast and accurate long-read sequencing by circular consensus sequencing (CCS) on the PacBio platform may greatly ...
Mascher, Martin, Wicker, Thomas, Jenkins, Jerry, Plott, Christopher, Lux, Thomas, Koh, Chu Shin, Ens, Jennifer, Gundlach, Heidrun, Boston, Lori B, Tulpová, Zuzana, Holden, Samuel, Hernández-Pinzón, Inmaculada, Scholz, Uwe, Mayer, Klaus F X, Spannagl, Manuel, Pozniak, Curtis J, Sharpe, Andrew G, Šimková, Hana, Moscou, Matthew J, Grimwood, Jane, Schmutz, Jeremy, Stein, Nils   +21 more
openaire   +6 more sources