Results 21 to 30 of about 680,559 (291)

Long-read sequencing settings for efficient structural variation detection based on comprehensive evaluation

BMC Bioinformatics, 2021
Background With the rapid development of long-read sequencing technologies, it is possible to reveal the full spectrum of genetic structural variation (SV).
Tao Jiang, Shiqi Liu, Shuqi Cao, Yadong Liu, Zhe Cui, Yadong Wang, Hongzhe Guo   +6 more
doaj   +1 more source

Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes

mSystems, 2022
Long-read sequencing offers the potential to improve metagenome assemblies and provide more robust assessments of microbial community composition and function than short-read sequencing.
N. V. Patin, K. D. Goodwin
doaj   +1 more source

Long-read sequencing data analysis for yeasts [PDF]

Nature Protocols, 2018
Long-read sequencing technologies have become increasingly popular due to their strengths in resolving complex genomic regions. As a leading model organism with small genome size and great biotechnological importance, the budding yeast Saccharomyces cerevisiae has many isolates currently being sequenced with long reads.
Yue, Jia-Xing, Liti, Gianni
openaire   +3 more sources

Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies

Microbiome, 2023
Background Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches.
Luis H. Orellana, Karen Krüger, Chandni Sidhu, Rudolf Amann   +3 more
doaj   +1 more source

Discovery of large genomic inversions using long range information. [PDF]

, 2017
BackgroundAlthough many algorithms are now available that aim to characterize different classes of structural variation, discovery of balanced rearrangements such as inversions remains an open problem.
Alkan, Can, Amemiya, Chris T, Antonacci, Francesca, Chiatante, Giorgia, Eichler, Evan E, Eslami Rasekh, Marzieh, Miroballo, Mattia, Tang, Joyce, Ventura, Mario   +8 more
core   +3 more sources

Ultra-accurate microbial amplicon sequencing with synthetic long reads

Microbiome, 2021
Background Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing.
Benjamin J. Callahan, Dmitry Grinevich, Siddhartha Thakur, Michael A. Balamotis, Tuval Ben Yehezkel   +4 more
doaj   +1 more source

Migrating to Long-Read Sequencing for Clinical Routine TKI Resistance Mutation Screening

Cancer Informatics, 2022
Objective: The aim of this project was to implement long-read sequencing for BCR-ABL1 TKI resistance mutation screening in a clinical setting for patients undergoing treatment for chronic myeloid leukemia.
Wesley Schaal, Adam Ameur, Ulla Olsson-Strömberg, Monica Hermanson, Lucia Cavelier, Ola Spjuth   +5 more
doaj   +1 more source

DiBELLA: Distributed long read to long read alignment [PDF]

, 2019
We present a parallel algorithm and scalable implementation for genome analysis, specifically the problem of finding overlaps and alignments for data from "third generation" long read sequencers [29]. While long sequences of DNA offer enormous advantages
Buluç, A, Ellis, M, Guidi, G, Oliker, L, Yelick, K   +4 more
core   +2 more sources

Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing [PDF]

, 2017
Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventional methods, less GC bias, and the ability to read DNA base modifications.
Henley, Robert Y., Jadhav, Vivek, Korlach, Jonas, Larkin, Joseph, Wanunu, Meni   +4 more
core   +1 more source

Long‐fragment targeted capture for long‐read sequencing of plastomes

Applications in Plant Sciences, 2019
Premise Third‐generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics.
Kevin Bethune, Cédric Mariac, Marie Couderc, Nora Scarcelli, Sylvain Santoni, Morgane Ardisson, Jean‐François Martin, Rommel Montúfar, Valentin Klein, François Sabot, Yves Vigouroux, Thomas L. P. Couvreur   +11 more
doaj   +1 more source