Results 1 to 10 of about 329,467 (113)

INSurVeyor: improving insertion calling from short read sequencing data [PDF]

Nature Communications, 2023
Insertions are one of the major types of structural variations and are defined as the addition of 50 nucleotides or more into a DNA sequence. Several methods exist to detect insertions from next-generation sequencing short read data, but they generally ...
Ramesh Rajaby, Dong-Xu Liu, Chun Hang Au, Yuen-Ting Cheung, Amy Yuet Ting Lau, Qing-Yong Yang, Wing-Kin Sung   +6 more
doaj   +2 more sources

Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies [PDF]

Microbiome, 2023
Background Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches.
Luis H. Orellana, Karen Krüger, Chandni Sidhu, Rudolf Amann   +3 more
doaj   +2 more sources

A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge [PDF]

mSystems
Driven by the increasing scale of microbiome studies and the rise of large, continuously expanding population cohorts, the volume of sequencing data is growing rapidly.
Kinga Zielińska, Kateryna Pantiukh, Pawel P. Łabaj, Tomasz Kosciolek, Elin Org   +4 more
doaj   +2 more sources

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

African Journal of Laboratory Medicine, 2020
No abstract available.
Boluwatife A. Adewale
doaj   +3 more sources

Oxford nanopore long-read sequencing enables the generation of complete bacterial and plasmid genomes without short-read sequencing [PDF]

Frontiers in Microbiology, 2023
IntroductionGenome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while long-read sequencing can obtain ...
Wenxuan Zhao, Wenxuan Zhao, Wei Zeng, Wei Zeng, Bo Pang, Ming Luo, Yao Peng, Jialiang Xu, Biao Kan, Biao Kan, Zhenpeng Li, Xin Lu   +11 more
doaj   +2 more sources

SeqEntropy: genome-wide assessment of repeats for short read sequencing. [PDF]

PLoS ONE, 2013
BACKGROUND: Recent studies on genome assembly from short-read sequencing data reported the limitation of this technology to reconstruct the entire genome even at very high depth coverage. We investigated the limitation from the perspective of information
Hsueh-Ting Chu, William W L Hsiao, Theresa T H Tsao, D Frank Hsu, Chaur-Chin Chen, Sheng-An Lee, Cheng-Yan Kao   +6 more
doaj   +1 more source

Long-Read Sequencing Annotation of the Transcriptome in DNA-PK Inactivated Cells

Frontiers in Oncology, 2022
The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) with a Ku70/Ku80 heterodimer constitutes the intact DNA-PK kinase, which is an upstream component of the DNA repair machinery that signals the DNA damage, orchestrates the DNA repair, and ...
Liwei Song, Liwei Song, Mengjun Yu, Renjing Jin, Meng Gu, Ziyu Wang, Dailun Hou, Shaofa Xu, Jinghui Wang, Jinghui Wang, Teng Ma   +10 more
doaj   +1 more source

Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes

mSystems, 2022
Long-read sequencing offers the potential to improve metagenome assemblies and provide more robust assessments of microbial community composition and function than short-read sequencing.
N. V. Patin, K. D. Goodwin
doaj   +1 more source

Investigating the Performance of Oxford Nanopore Long-Read Sequencing with Respect to Illumina Microarrays and Short-Read Sequencing. [PDF]

Int J Mol Sci
Santos R, Lee H, Williams A, Baffour-Kyei A, Lee SH, Troakes C, Al-Chalabi A, Breen G, Iacoangeli A.   +8 more
europepmc   +2 more sources

Sub genomic analysis of SARS-CoV-2 using short read amplicon-based sequencing

Frontiers in Genetics, 2023
The novel coronavirus disease 2019 (COVID-19) pandemic poses a serious public health risk. In this report, we present a modified sequencing workflow using short tiling (280bp) amplicons library preparation method paired with Illumina’s iSeq100 desktop ...
Lian Chye Winston Koh, Lian Chye Winston Koh, Yiqi Seow, Yiqi Seow, Kiat Whye Kong, Ming Li Lalita Lau, Shoban Krishna Kumar, Gabriel Yan, Chun Kiat Lee, Benedict Yan, Paul Anantharajah Tambyah, Shawn Hoon   +11 more
doaj   +1 more source