Results 11 to 20 of about 5,590,230 (287)
Unlocking short read sequencing for metagenomics.
PLoS ONE, 2010 BackgroundDifferent high-throughput nucleic acid sequencing platforms are currently available but a trade-off currently exists between the cost and number of reads that can be generated versus the read length that can be achieved.Methodology/principal ...
Sébastien Rodrigue +6 more
doaj +7 more sources
An analysis of the feasibility of short read sequencing [PDF]
Nucleic Acids Research, 2005 Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length.
Whiteford, N. +7 more
core +9 more sources
Short Read Mapping for Exome Sequencing
, 2013 Mapping short reads to the reference genome is very often the prerequisite for applications utilizing the next-generation sequencing technologies. A dozen of software tools developed for this purpose have been widely used. But many practical issues remained when utilizing them to build a computational pipeline for downstream analyses.
Zhang, X +4 more
openaire +5 more sources
Haplotype-Phased Synthetic Long Reads from Short-Read Sequencing. [PDF]
PLoS ONE, 2016 Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences.
James A Stapleton +12 more
doaj +6 more sources
, 2011 of EMIRGE: reconstruction of full-length ribosomal genes from microbial community short read sequencing ...
Christopher Miller (1120) +4 more
core +10 more sources
, 2022 Alternative splicing can lead to distinct protein isoforms. These can have different functions in specific cells and tissues or in different developmental stages.
Aidan P. Tay (1538278) +4 more
core +4 more sources
Impact of short-read sequencing on the misassembly of a plant genome. [PDF]
BMC Genomics, 2021 Abstract Background: Availability of plant genome sequences has led to significant advances. However, with few exceptions, the great majority of existing genome assemblies are derived from short read sequencing technologies with highly uneven read coverages indicative of sequencing and assembly issues that could significantly impact any ...
Wang P, Meng F, Moore BM, Shiu SH.
europepmc +5 more sources
Short read sequence typing (SRST): multi-locus sequence types from short reads [PDF]
BMC Genomics, 2012 Abstract Background Multi-locus sequence typing (MLST) has become the gold standard for population analyses of bacterial pathogens. This method focuses on the sequences of a small number of loci (usually seven) to divide the population and is simple, robust and facilitates comparison of results between laboratories ...
Inouye, M +3 more
openaire +4 more sources
Estimation of sequencing error rates in short reads [PDF]
BMC Bioinformatics, 2012 Abstract Background Short-read data from next-generation sequencing technologies are now being generated across a range of research projects. The fidelity of this data can be affected by several factors and it is important to have simple and reliable approaches for monitoring it at the level of individual experiments.
Blades, Natalie +4 more
openaire +5 more sources
Microindel detection in short-read sequence data [PDF]
Bioinformatics, 2010 Abstract Motivation: Several recent studies have demonstrated the effectiveness of resequencing and single nucleotide variant (SNV) detection by deep short-read sequencing platforms. While several reliable algorithms are available for automated SNV detection, the automated detection of microindels in deep short-read data presents a new ...
Peter M. Krawitz +5 more
openaire +5 more sources