Unlocking short read sequencing for metagenomics.
PLoS ONE, 2010 BackgroundDifferent high-throughput nucleic acid sequencing platforms are currently available but a trade-off currently exists between the cost and number of reads that can be generated versus the read length that can be achieved.Methodology/principal ...
Sébastien Rodrigue +6 more
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INSurVeyor: improving insertion calling from short read sequencing data [PDF]
Nature Communications, 2023 Insertions are one of the major types of structural variations and are defined as the addition of 50 nucleotides or more into a DNA sequence. Several methods exist to detect insertions from next-generation sequencing short read data, but they generally ...
Ramesh Rajaby +6 more
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Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies [PDF]
Microbiome, 2023 Background Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches.
Luis H. Orellana +3 more
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A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge [PDF]
mSystemsDriven by the increasing scale of microbiome studies and the rise of large, continuously expanding population cohorts, the volume of sequencing data is growing rapidly.
Kinga Zielińska +4 more
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Oxford nanopore long-read sequencing enables the generation of complete bacterial and plasmid genomes without short-read sequencing [PDF]
Frontiers in Microbiology, 2023 IntroductionGenome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while long-read sequencing can obtain ...
Wenxuan Zhao +11 more
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Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years? [PDF]
African Journal of Laboratory Medicine, 2020 No abstract available.
Boluwatife A. Adewale
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SeqEntropy: genome-wide assessment of repeats for short read sequencing. [PDF]
PLoS ONE, 2013 BACKGROUND: Recent studies on genome assembly from short-read sequencing data reported the limitation of this technology to reconstruct the entire genome even at very high depth coverage. We investigated the limitation from the perspective of information
Hsueh-Ting Chu +6 more
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Haplotype-Phased Synthetic Long Reads from Short-Read Sequencing. [PDF]
PLoS ONE, 2016 Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences.
James A Stapleton +12 more
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Whole-genome sequencing and assembly with high-throughput, short-read technologies.
PLoS ONE, 2007 While recently developed short-read sequencing technologies may dramatically reduce the sequencing cost and eventually achieve the $1000 goal for re-sequencing, their limitations prevent the de novo sequencing of eukaryotic genomes with the standard ...
Andreas Sundquist +4 more
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Using short read sequencing to characterise balanced reciprocal translocations in pigs [PDF]
BMC Genomics, 2020 Background A balanced constitutional reciprocal translocation (RT) is a mutual exchange of terminal segments of two non-homologous chromosomes without any loss or gain of DNA in germline cells.
Aniek C. Bouwman +4 more
doaj +3 more sources