Results 31 to 40 of about 4,886,903 (295)

Short read Illumina data for the de novo assembly of a non-model snail species transcriptome (Radix balthica, Basommatophora, Pulmonata), and a comparison of assembler performance [PDF]

, 2011
Background: Until recently, read lengths on the Solexa/Illumina system were too short to reliably assemble transcriptomes without a reference sequence, especially for non-model organisms.
Wheat, Christopher W., Markus Pfenninger, Rotter, Björn, Pfenninger, Markus, Björn Rotter, Christopher W Wheat, Nicolas Krezdorn, Feldmeyer, Barbara, Krezdorn, Nicolas, Rotter, Bjoern, Barbara Feldmeyer   +10 more
core   +2 more sources

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

, 2009
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD, Veenendaal, A., Johan T den Dunnen, Martien AM Groenen, Bert W Dibbits, Thomas FC Chin-A-Woeng, Dunnen, J.T., den, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Chin-A-Woeng, T.F.C., Dibbits Bert W, Hindrik HD Kerstens, Richard PMA Crooijmans, Veenendaal Albertine, den Dunnen Johan T, Chin-A-Woeng Thomas FC, Crooijmans, R.P.M.A., Albertine Veenendaal, Groenen Martien AM, Crooijmans Richard PMA, den Dunnen, J.T.   +21 more
core   +1 more source

Assembling genomes using short-read sequencing technology [PDF]

Genome Biology, 2010
Gigabase-scale genome assemblies are now feasible using short-read sequencing technology, bringing the cost of such projects below the million-dollar mark.
Jackman, Shaun D, Birol, İnanç
openaire   +2 more sources

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan, Eliza Xin Pei Ho, Andreas Wilm (7951), Hibberd, Martin Lloyd, Louie Low (400408), Nagarajan, Niranjan, Eliza Xin Pei Ho (400407), Ong, Swee Hoe, Christophe Lay (400406), Martin Lloyd Hibberd (259920), Low, Louie, Kukkillaya, Vinutha Uppoor, Andreas Wilm, Martin Lloyd Hibberd, Lay, Christophe, Niranjan Nagarajan (131213), Wilm, Andreas, Ho, Eliza Xin Pei, Vinutha Uppoor Kukkillaya (400405), Swee Hoe Ong, Louie Low, Swee Hoe Ong (400404), Vinutha Uppoor Kukkillaya, Christophe Lay   +23 more
core   +1 more source

Quantifying single nucleotide variant detection sensitivity in exome sequencing [PDF]

, 2013
BACKGROUND: The targeted capture and sequencing of genomic regions has rapidlydemonstrated its utility in genetic studies. Inherent in this technology isconsiderable heterogeneity of target coverage and this is expected tosystematically impact our ...
Taylor, Martin S.; id_orcid, Andrew P Jackson, Alison M Meynert, Jackson, Andrew, Bicknell, Louise, Martin S Taylor, Matthew E Hurles, Meynert, Alison, Louise S Bicknell   +8 more
core   +1 more source

Sequencing of natural strains of Arabidopsis thaliana with short reads [PDF]

Genome Research, 2008
Whole-genome hybridization studies have suggested that the nuclear genomes of accessions (natural strains) of Arabidopsis thaliana can differ by several percent of their sequence. To examine this variation, and as a first step in the 1001 Genomes Project for this species, we produced 15- to ...
Ossowski, Stephan, Schneeberger, Korbinian, Clark, Richard M, Lanz, Christa, Warthmann, Norman, Weigel, Detlef   +5 more
openaire   +4 more sources

A Review on Sequence Alignment Algorithms for Short Reads Based on Next-Generation Sequencing [PDF]

IEEE Access, 2020
With recent advances in next-generation sequencing (NGS) technology, large volumes of data have been produced in the form of short reads. Sequence assembly involves using initial short reads to produce progressively longer contigs, and then using scaffolds to produce the final sequence.
Jeongkyu Kim, Mingeun Ji, Gangman Yi
openaire   +2 more sources

Special features of RAD Sequencing data:implications for genotyping [PDF]

, 2012
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Fuentes-Utrilla, Pablo, Blaxter, Mark L, Pablo Fuentes-utrilla, Karim Gharbi, Davey, John W, Cezard, Timothée, John W. Davey, Gharbi, Karim, Pablo Fuentes‐Utrilla, Cathlene Eland, Timothée Cezard, Mark L. Blaxter, Eland, Cathlene   +12 more
core   +1 more source

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

BMC Microbiology, 2021
Background Species-level genetic characterization of complex bacterial communities has important clinical applications in both diagnosis and treatment. Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the ...
Yoshiyuki Matsuo, Shinnosuke Komiya, Yoshiaki Yasumizu, Yuki Yasuoka, Katsura Mizushima, Tomohisa Takagi, Kirill Kryukov, Aisaku Fukuda, Yoshiharu Morimoto, Yuji Naito, Hidetaka Okada, Hidemasa Bono, So Nakagawa, Kiichi Hirota   +13 more
doaj   +1 more source

POPULATION SEQUENCING USING SHORT READS: HIV AS A CASE STUDY [PDF]

Biocomputing 2008, 2007
Despite many drawbacks, traditional sequencing technologies have proven to be invaluable in modern medical research, even when the targeted genomes are highly variable. While it is often known in such cases that multiple slightly different sequences are present in the analyzed sample in concentrations that vary dramatically, the traditional techniques ...
Vladimir Jojic, Tomer Hertz, Nebojsa Jojic   +2 more
openaire   +3 more sources