Results 51 to 60 of about 5,590,230 (287)

Revealing co-infections in pneumonia: A case report on advancing diagnosis with metagenomic sequencing technologies

Journal of Infection and Public Health
Pneumonia, a frequent and serious complication in kidney transplant recipients, is significantly increased by long-term immunosuppressive medication. This case report details a patient with lung cavitation, in which conventional microbiological methods ...
Suzan Alzeer, Reem S. Almaghrabi, Amna Magrashi, Turki Alzahrani, Razan Bakheet, Abdulmohsen Alqasabi, Hamsa Tayeb   +6 more
doaj   +1 more source

Multi-Platform Sequencing Approach Reveals a Novel Transcriptome Profile in Pseudorabies Virus

Frontiers in Microbiology, 2018
Third-generation sequencing is an emerging technology that is capable of solving several problems that earlier approaches were not able to, including the identification of transcripts isoforms and overlapping transcripts. In this study, we used long-read
Norbert Moldován, Dóra Tombácz, Attila Szűcs, Zsolt Csabai, Michael Snyder, Zsolt Boldogkői   +5 more
doaj   +1 more source

Identification of stress-related genes by co-expression network analysis based on the improved turbot genome

Scientific Data, 2022
Measurement(s) whole genome sequencing Technology Type(s) PacBio long-read and Illumina short-read sequencing ...
Xi-wen Xu, Weiwei Zheng, Zhen Meng, Wenteng Xu, Yingjie Liu, Songlin Chen   +5 more
doaj   +1 more source

A multi-platform reference for somatic structural variation detection

Cell Genomics, 2022
Summary: Accurate detection of somatic structural variation (SV) in cancer genomes remains a challenging problem. This is in part due to the lack of high-quality, gold-standard datasets that enable the benchmarking of experimental approaches and ...
Jose Espejo Valle-Inclan, Nicolle J.M. Besselink, Ewart de Bruijn, Daniel L. Cameron, Jana Ebler, Joachim Kutzera, Stef van Lieshout, Tobias Marschall, Marcel Nelen, Peter Priestley, Ivo Renkens, Margaretha G.M. Roemer, Markus J. van Roosmalen, Aaron M. Wenger, Bauke Ylstra, Remond J.A. Fijneman, Wigard P. Kloosterman, Edwin Cuppen   +17 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Sequencing aided by mutagenesis facilitates the de novo sequencing of megabase DNA fragments by short read lengths

, 2007
"Sequencing by synthesis" (SBS) is a rapidly emerging high-throughput low-cost sequencing technology, and is one of the front-runners in the race to the $10,000 genome.
Mitchelson, K. R., Hawkes, David B., Cochran, Duncan A.E., David B. Hawkes, Adams, Peter, Adams, P., Bryant, D. E., Darryn E. Bryant, Keith, J. M., Carter, J. C., Keith R. Mitchelson, Hawkes D. B., Bryant, Darryn E., Duncan A.E. Cochran, Jonathan M. Keith, Carter, Jacinta C., Jacinta C. Carter, Mitchelson, Keith R., Keith, Macgregor, Cochran, D. A. E., Peter Adams   +20 more
core   +1 more source

A report on the 2009 SIG on short read sequencing and algorithms (Short-SIG) [PDF]

, 2009
Brudno M, Medvedev P, Stoye J, De La Vega FM. A report on the 2009 SIG on short read sequencing and algorithms (Short-SIG). BIOINFORMATICS.
M. Brudno, Paul Medvedev, Jens Stoye, Brudno, Michael, Francisco M. De La Vega, P. Medvedev, J. Stoye, Stoye, Jens ; https://orcid.org/, Medvedev, Paul, De La Vega, Francisco M., F. M. De La Vega, Michael Brudno   +11 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Evaluating the accuracy of Listeria monocytogenes assemblies from quasimetagenomic samples using long and short reads

BMC Genomics, 2021
Background Whole genome sequencing of cultured pathogens is the state of the art public health response for the bioinformatic source tracking of illness outbreaks.
Seth Commichaux, Kiran Javkar, Padmini Ramachandran, Niranjan Nagarajan, Denis Bertrand, Yi Chen, Elizabeth Reed, Narjol Gonzalez-Escalona, Errol Strain, Hugh Rand, Mihai Pop, Andrea Ottesen   +11 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source