Results 41 to 50 of about 5,590,230 (287)

Comparison of short-read and long-read sequencing of TprK.

, 2020
Comparison of the determination of identical amino acid sequences in each of the variable regions of TprK through short-read (Illumina) and long-read (PacBio CCS) sequencing approaches for A) the initial strain, UW-148B and B) the reinfecting strain, UW ...
Amin Addetia (8201538), Lauren C. Tantalo (8677368), Christina M. Marra (8677374), Hong Xie (133048), Meei-Li Huang (342097), Alexander L. Greninger (8677377), Michelle J. Lin (8677371)   +6 more
core   +1 more source

Short- and long-read RNA-seq to capture chromatin-enriched RNA.

, 2023
A. Schematic illustrating the nuclear fractionation of cells to isolate chromatin-enriched RNA (cheRNA) then subjected to short- and long-read sequencing.
Johnathon M. Hall (8431497), Alexander J. Ruthenburg (4585114), Amoldeep S. Kainth (17291225), Gabriela A. Haddad (17291228)   +3 more
core   +1 more source

Presentation_1_Oxford nanopore long-read sequencing enables the generation of complete bacterial and plasmid genomes without short-read sequencing.PDF

, 2023
IntroductionGenome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while long-read sequencing can obtain ...
Wenxuan Zhao (4615180), Zhenpeng Li (553533), Ming Luo (13872), Jialiang Xu (1424821), Yao Peng (1928524), Xin Lu (24821), Bo Pang (164741), Wei Zeng (126658), Biao Kan (107255)   +8 more
core   +1 more source

Testing assembly strategies of Francisella tularensis genomes to infer an evolutionary conservation analysis of genomic structures

BMC Genomics, 2021
Background We benchmarked sequencing technology and assembly strategies for short-read, long-read, and hybrid assemblers in respect to correctness, contiguity, and completeness of assemblies in genomes of Francisella tularensis.
Kerstin Neubert, Eric Zuchantke, Robert Maximilian Leidenfrost, Roebbe Wuenschiers, Josephine Grützke, Burkhard Malorny, Holger Brendebach, Sascha Al Dahouk, Timo Homeier, Helmut Hotzel, Knut Reinert, Herbert Tomaso, Anne Busch   +12 more
doaj   +1 more source

Table_1_Oxford nanopore long-read sequencing enables the generation of complete bacterial and plasmid genomes without short-read sequencing.DOCX

, 2023
IntroductionGenome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while long-read sequencing can obtain ...
Wenxuan Zhao (4615180), Zhenpeng Li (553533), Ming Luo (13872), Jialiang Xu (1424821), Yao Peng (1928524), Xin Lu (24821), Bo Pang (164741), Wei Zeng (126658), Biao Kan (107255)   +8 more
core   +1 more source

Software for pre-processing Illumina next-generation sequencing short read sequences [PDF]

Source Code for Biology and Medicine, 2014
When compared to Sanger sequencing technology, next-generation sequencing (NGS) technologies are hindered by shorter sequence read length, higher base-call error rate, non-uniform coverage, and platform-specific sequencing artifacts. These characteristics lower the quality of their downstream analyses, e.g.
Chuming Chen, Sari S. Khaleel, Hongzhan Huang, Cathy H. Wu   +3 more
openaire   +3 more sources

Linked-read sequencing for detecting short tandem repeat expansions

Scientific Reports, 2022
Detection of short tandem repeat (STR) expansions with standard short-read sequencing is challenging due to the difficulty in mapping multicopy repeat sequences.
Readman Chiu, Indhu-Shree Rajan-Babu, Inanc Birol, Jan M. Friedman   +3 more
doaj   +1 more source

Table_2_Oxford nanopore long-read sequencing enables the generation of complete bacterial and plasmid genomes without short-read sequencing.DOCX

, 2023
IntroductionGenome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while long-read sequencing can obtain ...
Zhenpeng Li (553533), Biao Kan, Ming Luo, Ming Luo (13872), Jialiang Xu (1424821), Xin Lu, Xin Lu (24821), Bo Pang (164741), Wenxuan Zhao (4615180), Bo Pang, Yao Peng, Wenxuan Zhao, Zhenpeng Li, Yao Peng (1928524), Jialiang Xu, Wei Zeng, Wei Zeng (126658), Biao Kan (107255)   +17 more
core   +1 more source

Can long-read sequencing tackle the barriers, which the next-generation could not? A review

Pathology and Oncology Research
The large-scale heterogeneity of genetic diseases necessitated the deeper examination of nucleotide sequence alterations enhancing the discovery of new targeted drug attack points.
Nikolett Szakállas, Barbara K. Barták, Gábor Valcz, Gábor Valcz, Zsófia B. Nagy, István Takács, Béla Molnár   +6 more
doaj   +1 more source

Detecting Expansions of Tandem Repeats in Cohorts Sequenced with Short-Read Sequencing Data [PDF]

The American Journal of Human Genetics, 2018
Repeat expansions cause more than 30 inherited disorders, predominantly neurogenetic. These can present with overlapping clinical phenotypes, making molecular diagnosis challenging. Single-gene or small-panel PCR-based methods can help to identify the precise genetic cause, but they can be slow and costly and often yield no result.
Tankard, R.M., Bennett, M.F., Degorski, P., Delatycki, M.B., Lockhart, P.J., Bahlo, M.   +5 more
openaire   +2 more sources