Results 81 to 90 of about 5,590,230 (287)

Discovery of barley miRNAs through deep sequencing of short reads [PDF]

BMC Genomics, 2011
Abstract Background MicroRNAs are important components of the regulatory network of biological systems and thousands have been discovered in both animals and plants. Systematic investigations performed in species with sequenced genomes such as Arabidopsis, rice, poplar and Brachypodium have provided insights into the ...
Schreiber, A., Shi, B., Huang, C., Langridge, P., Baumann, U.   +4 more
openaire   +4 more sources

Statistic of Short-read and Long-read Data of Sengon (Falcataria moluccana).docx

, 2022
This project contains data files results from short-read and long-read sequencing technology of Sengon (Falcataria moluccana)
Deden Derajat Matra (8840792), Ulfah J. Siregar (5999054), Vilda Puji Dini Anita (14180708)   +2 more
core   +1 more source

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Short-read, high-throughput sequencing technology for STR genotyping

BioTechniques, 2012
DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques for variable-length classification of STRs are universally utilized, but are limited in that they have relatively low throughput and do not yield nucleotide sequence information.
Daniel M, Bornman, Mark E, Hester, Jared M, Schuetter, Manjula D, Kasoji, Angela, Minard-Smith, Curt A, Barden, Scott C, Nelson, Gene D, Godbold, Christine H, Baker, Boyu, Yang, Jacquelyn E, Walther, Ivan E, Tornes, Pearlly S, Yan, Benjamin, Rodriguez, Ralf, Bundschuh, Michael L, Dickens, Brian A, Young, Seth A, Faith   +17 more
openaire   +2 more sources

Identification of avian W-linked contigs by short-read sequencing [PDF]

BMC Genomics, 2012
The female-specific W chromosomes and male-specific Y chromosomes have proven difficult to assemble with whole-genome shotgun methods, creating a demand for new approaches to identify sequence contigs specific to these sex chromosomes. Here, we develop and apply a novel method for identifying sequences that are W-specific.Using the Illumina Genome ...
Chen, Nancy, Bellott, Daniel W., Clark, Andrew G, Page, David C   +3 more
openaire   +5 more sources

Choosing the most suitable NGS technology to combine with a standardized viral enrichment protocol for obtaining complete avian orthoreovirus genomes from metagenomic samples

Frontiers in Bioinformatics
Since viruses are obligate intracellular pathogens, sequencing their genomes results in metagenomic data from both the virus and the host. Virology researchers are constantly seeking new, cost-effective strategies and bioinformatic pipelines for the ...
Sonsiray Álvarez-Narváez, Sonsiray Álvarez-Narváez, Telvin L. Harrell, Islam Nour, Sujit K. Mohanty, Steven J. Conrad   +5 more
doaj   +1 more source

MOST: a modified MLST typing tool based on short read sequencing [PDF]

PeerJ, 2016
Multilocus sequence typing (MLST) is an effective method to describe bacterial populations. Conventionally, MLST involves Polymerase Chain Reaction (PCR) amplification of housekeeping genes followed by Sanger DNA sequencing.
Rediat Tewolde, Timothy Dallman, Ulf Schaefer, Carmen L. Sheppard, Philip Ashton, Bruno Pichon, Matthew Ellington, Craig Swift, Jonathan Green, Anthony Underwood   +9 more
doaj   +2 more sources

Epigenetic silencing of the liver‐specific lncRNA LUNAR promotes liver cancer progression via NOTCH activation

Molecular Oncology, EarlyView.
LUNAR is a liver‐specific long noncoding RNA (lncRNA) that is highly expressed in normal liver but becomes epigenetically silenced in hepatocellular carcinoma through promoter hypermethylation. Loss of LUNAR is associated with NOTCH activation, epithelial–mesenchymal transition, and metastasis, whereas restoring LUNAR restrains metastatic progression ...
Se Ha Jang, Hyung Seok Kim, Geum Ok Baek, Moon Gyeong Yoon, Su In Lee, Jee‐Yeong Jeong, Ji Eun Han, Soon Sun Kim, Jae Youn Cheong, Jung Woo Eun   +9 more
wiley   +1 more source

Investigating structural variant, indel and single nucleotide polymorphism differentiation between locally adapted Atlantic salmon populations

Evolutionary Applications
Genomic structural variants (SVs) are now recognized as an integral component of intraspecific polymorphism and are known to contribute to evolutionary processes in various organisms.
Laurie Lecomte, Mariann Árnyasi, Anne‐Laure Ferchaud, Matthew Kent, Sigbjørn Lien, Kristina Stenløkk, Florent Sylvestre, Louis Bernatchez, Claire Mérot   +8 more
doaj   +1 more source

Intrapatient tumour heterogeneity and clonal evolution in an autopsy study of metastatic salivary gland cancer

Molecular Oncology, EarlyView.
Tumour heterogeneity and clonal evolution of metastatic salivary gland cancer were evaluated in two patients with adenoid carcinoma and one patient with myoepithelial carcinoma. Radiology‐guided autopsy enabled multi‐region sampling (total samples n = 149), followed by whole‐genome sequencing and phylogenetic reconstruction (17 tumour samples, 4–7 per ...
Gerben Lassche, Niels J. van Ruitenbeek, Charlotte Adang, Luke O'Gorman, Pui Yuen Lee, Willemijn M. Klein, Adriana C. H. van Engen‐van Grunsven, Jack A. Schalken, Sander Bervoets, Gerald W. Verhaegh, Carla M. L. van Herpen   +10 more
wiley   +1 more source