Results 81 to 90 of about 163,355 (309)

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Characterization of shRNA-β clones. [PDF]

, 2015
(A) Immunoblots showing the levels of NaK-β1 and NaK-α1 in Caki-1 cells stably transfected with scrambled shRNA (SCRAM) or shRNA against NaK-β1 (shRNA-β). Two clones (cl#1 and cl#2) were further studied. Actin was used as a loading control.
Thu P. Huynh (716782), Robert Damoiseaux (326975), Nicholas J. Petrelli (716786), Omar E. Franco (86997), Ayyappan K. Rajasekaran (598227), Simon W. Hayward (86998), Ryan McSpadden (716784), Seung J. Lee (297806), Sonali P. Barwe (716783), Stephen S. Grubbs (716785)   +9 more
core   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

shRNA-mediated rho-Gluc activity and thermodynamic stability. [PDF]

, 2016
The minimum free energy of shRNAs and their effect on rho-Gluc activity were plotted. Error bars are shown as SE.
Donald J. Zack (9715), Jun Wan (126294), Cindy Berlinicke (3564980), Anitha Yerrabelli (3564977), Tomohiro Masuda (14600), Alyssa Kallman (3564974), Jiang Qian (771)   +6 more
core   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

shRNA-mediated knockdown of Na_v1.1. [PDF]

, 2016
Lentiviral vectors were generated for two separate shRNAs targeting the rat Scn1a gene (sh-1 and sh-2). (A-B) Lentiviral vectors were tested in B50 neuroblastoma cells for efficient knockdown of the target gene.
Bryan W. Luikart (351588), Pierre-Pascal Lenck-Santini (526851), Alex C. Bender (3179583)   +2 more
core   +1 more source

Stromal‐Derived IL‐8 Promotes M2 Macrophage Polarization via the RhoA/MRTF‐A/SRF Transcriptional Axis to Impair CD8+ T Cell Cytotoxicity in Lung Cancer

Advanced Science, EarlyView.
In the recondite pulmonary neoplastic milieu, DESMIN+ cancer‐associated fibroblasts function as the paramount stromal source of IL‐8, precipitating a deleterious immunological metamorphosis by engaging CXCR2 receptors on the myeloid lineage. This paracrine stimulus triggers a mechanotransduction cascade characterized by RhoA‐dependent actin ...
Xuyu Gu, Qiyu Fang, Jia Yu, Ting Yu, Huashan Shi, Kaiqi Jin, Lei Jiang, Wentian Zhang   +7 more
wiley   +1 more source

Parameters of mitochondrial trafficking in neurons transfected with scrambled shRNA or Wfs1 shRNA. [PDF]

, 2016
Parameters of mitochondrial trafficking in neurons transfected with scrambled shRNA or Wfs1 shRNA.
Mailis Liiv (3151065), Michal Cagalinec (3151077), Allen Kaasik (504365), Zuzana Hodurova (3151083), Vinay Choubey (3151080), Annika Vaarmann (321196), Akbar Zeb (3151071), Malle Kuum (3151074), Vladimir Veksler (201015), Miriam Ann Hickey (3151068), Merle Mandel (578705), Dzhamilja Safiulina (504364), Eero Vasar (91530)   +12 more
core   +1 more source

FoxM1 is Upregulated in Osteosarcoma and Inhibition of FoxM1 Decreases Osteosarcoma Cell Proliferation, Migration, and Invasion

Cancer Management and Research, 2020
Xia Zhu,1,2 Kangyang Lu,1 Liyu Cao,1,3 Yong Hu,4 Yu Yin,1,3 Yongping Cai1,3 1Department of Pathology, School of Basic Medical Science, Anhui Medical University, Hefei 230032, People’s Republic of China; 2Department of Pathology, Huadong Hospital ...
Zhu X, Lu K, Cao L, Hu Y, Yin Y, Cai Y
doaj  

Criteria for effective design, construction, and gene knockdown by shRNA vectors

BMC Biotechnology, 2006
Background RNA interference (RNAi) technology is a powerful methodology recently developed for the specific knockdown of targeted genes. RNAi is most commonly achieved either transiently by transfection of small interfering (si) RNA oligonucleotides, or ...
Lich John D, Williams Kristi L, Iocca Heather A, Conti Brian J, Zhang Jinghua, Livingstone Laura R, Taxman Debra J, Ting Jenny, Reed William   +8 more
doaj   +1 more source