Results 71 to 80 of about 163,355 (309)
Molecular Oncology, EarlyView.We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source
Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source
The shRNA loop co-determine shRNA efficacy. [PDF]
, 2013 A. Schematic representation of the shRNA-loop selection experiment. DNA fragments encoding shRNA hairpins with randomised loop sequences of 7, 9, or 11 nucleotides in length were inserted into a retroviral expression cassette and packaged into viral ...
Jørgen Kjems (195388) +4 more
core +1 more source
Potentiation of oncolytic virotherapy with armed shRNA [PDF]
Cancer Biology & Therapy, 2009 Commentary to:Inhibition of renal cancer cell growth by oncolytic adenovirus armed short hairpin RNA targeting hTERT geneJun-Nian Zheng, Dong-Sheng Pei, Fang-Hao Sun, Bao-Fu Zhang, Xin-Yuan Liu, Jin-Fa Gu, Yan-Hua Liu, Xue-Li Hu, Li-Jun Mao, Ru-Min Wen, Jun-Jie Liu, Wang ...
Wei, Guo, Bingliang, Fang
openaire +2 more sources
Molecular Oncology, EarlyView.BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi +11 more
wiley +1 more source
Cancer Management and Research, 2020 Yakun Liu,1,2,* Yuanyuan Li,1,2,* Zhongyao Li,1,2 Chunyan Li,1– 3 Junying He,1– 3 Hui Bu1– 3 1Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, People’s Republic of China ...
Liu Y, Li Y, Li Z, Li C, He J, Bu H
doaj
Molecular Therapy: Nucleic Acids, 2017 RNAi by short hairpin RNA (shRNA) is a powerful tool not only for studying gene functions in various organisms, including mammals, but also for the treatment of severe disorders.
Mitsuhiro Machitani +5 more
doaj +1 more source
Inhibiting autophagy by shRNA knockdown [PDF]
Autophagy, 2013 A glance through Autophagy or any other journal in this field shows that it is very common to block autophagy by RNA interference-based knockdown of ATG mRNAs in mammalian cell lines. Our lab's experience is that this approach can easily make for failed experiments because good knockdown of even essential autophagy regulators does not necessarily mean ...
Leah, Staskiewicz +3 more
openaire +2 more sources