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[10] Determination of sialic acids
1994Publisher Summary This chapter describes isolation, purification, and characterization of monomeric sialic acids. There are two basic procedures for liberating sialic acids from glycosidic linkages—enzymatic and acid hydrolysis. In the former case, a variety of sialidases may be used, which differ in specificity for sialic acid linkage or species ...
Gerd Reuter, Roland Schauer
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Nonulosaminic Acid (Sialic Acid) in Protists
Nature, 1960NONULOSAMINIC ACID1, a group of simple and substituted 9-carbon sugars containing nitrogen, was thought restricted to mammals until it was found in the supernates of Escherichia coli and Citrobacter freundii cultures, but not in the supernates of Salmonella, Shigella, Pneumococcus or Bacillus cultures2.
Stuart A. Aaronson +3 more
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Metabolism of Sialic Acids [PDF]
, 1982 The wide occurrence of sialic acids in nature is an indication of their great biological importance. As is described in other chapters in this book, the identification of the sialic acids was pioneered in several laboratories, while the biosynthetic mechanism of sialic acid formation was elucidated essentially by the work of Roseman’s and Warren’s ...
Roland Schauer, Anthony P. Corfield
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Polarographic Examination of Sialic Acids
Nature, 1961WE have investigated systematically the polarographic wave of neuraminic acid derivatives with a dropping-mercury electrode (m = 2.86 mgm./sec., t = 2.98 sec.). The existence of a sialic acid wave was anticipated from the fact that structurally related substances such as pyruvic acid1,2 and fructose3,4 give well-defined polarographic waves.
Barbara Robert +2 more
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Fluorimetric assay of sialic acids
Analytical Biochemistry, 1976Abstract A fluorimetric assay has been developed for sialic acids in which sialic acids react with pyridoxamine to give fluorescent compounds in the presence of zinc ion and pyridine. This assay method is specific for unbound sialic acids and is a simple and sensitive procedure compared with the thiobarbituric acid assay of sialic acids.
Akira Hamada +3 more
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The distribution of sialic acids in nature
Comparative Biochemistry and Physiology, 1963Abstract 1. 1. The occurrence of sialic acids acids in nature has been studied. These acids are found in all species of Vertebrata, Cephalochorda, Hemichorda and Echinoderma tested. 2. 2. They are distributed sporadically in the phyla Platyhelminthes, Mollusca, Anthropoda and in bacteria.
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Histochemistry of Sialic Acids
1982The aim of this chapter will be to escort the reader through the maze of histochemical methodology available for the location and identification of sialic acids and their variants in tissue sections. We shall confine most of our discussion to those forms of sialic acid which can be demonstrated histochemically, the methodology by which they may be ...
C. F. A. Culling, P. E. Reid
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1982
The discovery and the widespread occurrence of the sialic acids in mammalian tissues (Blix 1936, Klenk 1941) has been correlated with a range of different biological functions (see chapter J) which continues to expand. Although the literature on sialic acid occurrence is large (Gottschalk 1960, Blix and Jeanloz 1969, Tuppy and Gottschalk 1972, Schauer ...
Roland Schauer, Anthony P. Corfield
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The discovery and the widespread occurrence of the sialic acids in mammalian tissues (Blix 1936, Klenk 1941) has been correlated with a range of different biological functions (see chapter J) which continues to expand. Although the literature on sialic acid occurrence is large (Gottschalk 1960, Blix and Jeanloz 1969, Tuppy and Gottschalk 1972, Schauer ...
Roland Schauer, Anthony P. Corfield
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Sialic acid and mucus rheology
Clinica Chimica Acta, 1981Mucus glycoproteins are high-molecular-mass proteins with many hundreds of oligosaccharide units. Each unit contains about S10 monosaccharide residues. Sialic acid is located at the terminals on carbohydrate chains. In humans sialic acid is represented by N-acetylneuraminic acid (NANA), but other sialic acids such as N-glycollylneuraminic acid or O ...
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