Results 71 to 80 of about 3,392,560 (298)

Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region

Microbial Cell Factories, 2020
Background Recombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant (gram-positive bacteria).
Kiavash Mirzadeh, Patrick J. Shilling, Rageia Elfageih, Alister J. Cumming, Huanhuan L. Cui, Maja Rennig, Morten H. H. Nørholm, Daniel O. Daley   +7 more
doaj   +1 more source

Calmodulin as a Key Regulator of Exosomal Signal Peptides

Cells, 2022
Signal peptides (SPs) and their fragments play important roles as biomarkers and substances with physiological functions in extracellular fluid. We previously reported that SP fragments were released into extracellular fluid via exosomes and bound to ...
Kenji Ono, Mikio Niwa, Hiromi Suzuki, Nahoko Bailey Kobayashi, Tetsuhiko Yoshida, Makoto Sawada   +5 more
doaj   +1 more source

The signal peptide of human preproendothelin‐1 [PDF]

FEBS Letters, 1991
Synthetic mRNAs were produced using either the complete coding sequence of a human preproendothelin‐1 cDNA clone or a truncated form in which the portion encoding the first 17 amino acids, representing a putative signal peptide for insertion into the endoplasmic reticulum, was replaced with a methionine codon.
Fabbrini, Maria Serena, Valsasina, Barbara, Nitti, Gianpaolo, Benatti, Luca, Vitale, Alessandro   +4 more
openaire   +2 more sources

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Development of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli

Scientific Reports, 2018
Targeting of recombinant proteins to the Escherichia coli periplasm is a desirable industrial processing tool to allow formation of disulphide bonds, aid folding and simplify recovery.
Tania Selas Castiñeiras, Steven G. Williams, Antony Hitchcock, Jeffrey A. Cole, Daniel C. Smith, Tim W. Overton   +5 more
doaj   +1 more source

A bovine CD18 signal peptide variant with increased binding activity to Mannheimia hemolytica leukotoxin [version 1; referees: 2 approved]

F1000Research, 2018
Background: Mannheimia haemolytica is the major bacterial infectious agent of bovine respiratory disease complex and causes severe morbidity and mortality during lung infections. M.
Aspen M. Workman, Carol G. Chitko-McKown, Timothy P. L. Smith, Gary L. Bennett, Theodore S. Kalbfleisch, Veronica Basnayake, Michael P. Heaton   +6 more
doaj   +1 more source

Natriuretic peptide receptor-C regulates coronary blood flow and prevents myocardial ischemia/reperfusion injury: novel cardioprotective role for endothelium-derived C-type natriuretic peptide [PDF]

, 2004
Background: Ischemia/reperfusion (I/R) injury complicates myocardial infarction and stroke by exacerbating tissue damage and increasing risk of mortality.
Ramona Scotl, Hobbs, Adrian, Ahluwalia, Amrita, Foster, Paul, Hobbs, A., Phd Amrita Ahluwalia, Foster, P., Adrian Hobbs, Phd Paul Foster, Scotland, R., Prescott, Craig, Scotland, Ramona, Ahluwalia, A., Phd Craig Prescott, Natriuretic Peptide, Prescott, C.   +15 more
core   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Design of signal peptide bombyxin and its effect on secretory expression efficiency and levels of Helicobacter pylori urease subunit B in silkworm cells and larvae

Brazilian Archives of Biology and Technology, 2015
This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori.The signal peptide bombyxin from B.
Xiao-lin Zhang, An-min Jiang, Zhong-you Ma, You-yi Xiong, Jin-feng Dou, Guo-liang Zhou, Mei-song Qin, Jian-fei Wang   +7 more
doaj   +1 more source