Results 111 to 120 of about 6,502,084 (338)

Protocol for CRISPR-Cas12a genome editing of protein tyrosine phosphatases in human pluripotent stem cells and functional β-like cell generation

STAR Protocols
Summary: Gene editing of human pluripotent stem cells is a promising approach for developing targeted gene therapies for metabolic diseases. Here, we present a protocol for generating a CRISPR-Cas12a gene knockout of protein tyrosine phosphatases in ...
Javier Negueruela, Valerie Vandenbempt, Stephanie Talamantes, Francisco Ribeiro-Costa, Mariana Nunes, André Dias, Mayank Bansal, Esteban N. Gurzov   +7 more
doaj   +1 more source

Length-dependent gene misexpression is associated with Alzheimer’s disease progression

Scientific Reports, 2017
Recent reports show transcription preference for long genes in neuronal tissues compared with non-neuronal tissues, and a gene-length dependent change in expression in the neurodevelopmental disease Rett syndrome (RTT).
Shahar Barbash, Thomas P. Sakmar
doaj   +1 more source

Novel Signal Transduction Pathway Utilized by Extracellular HSP70

Journal of Biological Chemistry, 2002
Recent studies have initiated a paradigm shift in the understanding of the function of heat shock proteins (HSP). It is now clear that HSP can and do exit mammalian cells, interact with cells of the immune system, and exert immunoregulatory effects.
A. Asea, M. Rehli, E. Kabingu, J. A. Boch, Olivia Baré, P. Auron, M. Stevenson, S. Calderwood   +7 more
semanticscholar   +1 more source

Metastasis on pause: How dormant tumor cells stay hidden within the tumor microenvironment and evade immune surveillance

Molecular Oncology, EarlyView.
Dormant cancer cells can hide in distant organs for years, evading treatment and the immune system. This review highlights how signals from the surrounding tissue and immune environment keep these cells inactive or trigger their reawakening. Understanding these mechanisms may help develop therapies to eliminate or control dormant cells and prevent ...
Kanishka Tiwary, Jose Javier Bravo‐Cordero   +1 more
wiley   +1 more source

Plasma hepcidin level is elevated by water immersion‐induced central fatigue via hepatic inflammatory response in male and female rats

Physiological Reports
Fatigue is a subjective phenomenon caused by physical or mental overexertion; however, its objective biomarkers specific to the types of fatigue remain unclear. Here, we examined whether plasma hepcidin levels, which are regulated by inflammation or iron
Takuro Karaushi, Toshifumi Ogawa, Hiroyori Fusagawa, Taiki Kudo, Yuito Inoue, Takashi Yamada, Nobutoshi Ichise, Tatsuya Sato, Noritsugu Tohse   +8 more
doaj   +1 more source

Signal transduction pathways in the clinical approach: where are we going? [PDF]

, 2016
invited ...
LO VASCO, VINCENZA RITA
core   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model

Communications Biology
Whether organ-specific regeneration is induced by organ-specific endothelial cells (ECs) remains unelucidated. The formation of white matter lesions due to chronic cerebral hypoperfusion causes cognitive decline, depression, motor dysfunction, and even ...
Yuichi Matsui, Fumitaka Muramatsu, Hajime Nakamura, Yoshimi Noda, Kinnosuke Matsumoto, Haruhiko Kishima, Nobuyuki Takakura   +6 more
doaj   +1 more source

Proteomic maps of breast cancer subtypes

Nature Communications, 2016
Breast cancers have been extensively studied at the genomic and transcriptomic levels in the hope of tailoring therapeutic regimens. Here the authors generate deep coverage proteomes from several clinical breast cancer samples, and use machine learning ...
Stefka Tyanova, Reidar Albrechtsen, Pauliina Kronqvist, Juergen Cox, Matthias Mann, Tamar Geiger   +5 more
doaj   +1 more source

Distinct functions of AKT isoforms in breast cancer: a comprehensive review

Cell Communication and Signaling, 2019
Background AKT, also known as protein kinase B, is a key element of the PI3K/AKT signaling pathway. Moreover, AKT regulates the hallmarks of cancer, e.g. tumor growth, survival and invasiveness of tumor cells. After AKT was discovered in the early 1990s,
Nico Hinz, Manfred Jücker
doaj   +1 more source