Results 201 to 210 of about 179,325 (263)

A Cholesterol Analogue for Cell‐Surface Enzyme Display

open access: yesAngewandte Chemie, EarlyView.
We report a non‐genetic strategy for bacterial cell surface enzyme immobilisation using cholesterol‐based artificial lipids containing Ni2+‐NTA binding groups that can integrate into biological membranes. The engineered cells can capture His‐tagged enzymes directly from cell lysates while preserving intracellular and surface activity, allowing for ...
Vasco F. Batista   +3 more
wiley   +1 more source

Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides by a Covalent Catch‐and‐Release Approach

open access: yesAngewandte Chemie, EarlyView.
A glycan having a hydrazide tag enables chemoenzymatic synthesis of HS oligosaccharides. The hydrazide can, under mild acidic conditions, react with an aldehyde‐containing resin to give an immobilized hydrazone that is stable under neutral conditions, allowing washing. The product can be released by transiminolysis using aqueous hydroxylamine to give a
Francesco Palmieri   +4 more
wiley   +1 more source
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Structure of silica gels

Physical Review Letters, 1991
The structure of silica hydrogels has been studied by elastic light scattering. Like colloidal silica gels and neutrally catalyzed aerogels, these gels show mass fractal behavior at length scales below a crossover length \ensuremath{\xi} and scatter like a spatially random distribution of fractal objects for length scales g\ensuremath{\xi}.
F. FERRI, B. J. FRISKEN, D. S. CANNELL
openaire   +3 more sources

Living bacteria in silica gels

Nature Materials, 2002
The encapsulation of enzymes within silica gels has been extensively studied during the past decade for the design of biosensors and bioreactors. Yeast spores and bacteria have also been recently immobilized within silica gels where they retain their enzymatic activity, but the problem of the long-term viability of whole cells in an inorganic matrix ...
Nadine, Nassif   +5 more
openaire   +2 more sources

Crystallization of macromolecules in silica gels

Acta Crystallographica Section D Biological Crystallography, 1994
Procedures are described for the crystallization of proteins, nucleic acids and viruses in a silica-gel matrix using otherwise standard reagents and conditions. Methods are given based on both vapor diffusion in a sitting drop and liquid-liquid diffusion.
R, Cudney, S, Patel, A, McPherson
openaire   +2 more sources

Chemical modification of silica gels

Journal of Sol-Gel Science and Technology, 1997
Highly porous pure and polyethyleneglycol (PEG)-doped silica gels have been prepared from tetraethyl orthosilicate (TEOS) in ethanol using NH4OH base-catalysis. Addition of PEG to the SiO2-systemincreases the average particle size and most frequent pore diameter of the resultant gels while their total surface areadecreases.
M.S.W. Vong, N. Bazin, P.A. Sermon
openaire   +1 more source

Adhesion of Gels by Silica Particle

The Journal of Physical Chemistry B, 2014
In this study, a method for achieving adhesion between two positively charged gels with high mechanical strength was developed. By utilizing a silica particle dispersion as a binder, the gels easily adhered to each other and remained stable for up to 11 days when immersed in aqueous solution.
Abe, H.   +3 more
openaire   +2 more sources

A comparison of the chromatographic properties of silica gel and silicon hydride modified silica gels

Journal of Chromatography A, 2012
The retention properties of a silica gel column and a type C silica (silicon hydride) column for bases, sugars and polar acids were compared in hydrophilic interaction chromatography (HILIC) mode with formic acid or ammonium acetate as aqueous phase modifiers.
Bawazeer, Saud   +4 more
openaire   +4 more sources

Gel-Silica Science

Annual Review of Materials Research, 1990
Sol-gel techniques can be used to produce two new types of optical silicas, termed Type V for the full density material and Type VI for the optically transparent porous material. This paper summarizes the processing differences between these six types of commercial silicas.
L L Hench, W Vasconcelos
openaire   +2 more sources

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