Results 111 to 120 of about 5,167,602 (281)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

A lower bound on the number of finite simple groups

open access: yesInternational Journal of Mathematics and Mathematical Sciences, 1980
Let S(n ...
Michael E. Mays
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Finite simple groups of low rank: Hurwitz generation and (2,3)-generation [PDF]

open access: yesInternational Journal of Group Theory, 2015
‎Let us consider the set of non-abelian finite simple groups which‎ ‎admit non-trivial irreducible projective representations of degree ≤7 over‎ ‎an algebraically closed field F of characteristic p≥0 ‎.
Marco Pellegrini, Maria Tamburini
doaj  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Quasirecognition by prime graph of finite simple groups ${}^2D_n(3)$ [PDF]

open access: yesInternational Journal of Group Theory, 2014
Let $G$ be a finite group. In [Ghasemabadi et al., characterizations of the simple group ${}^2D_n(3)$ by prime graph and spectrum, Monatsh Math., 2011] it is proved that if $n$ is odd, then ${}^2D _n(3)$ is recognizable by prime graph and also by ...
Behrooz Khosravi , Hossein Moradi
doaj  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

open access: yesFEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa   +2 more
wiley   +1 more source

Simple compact quantum groups I

open access: yesJournal of Functional Analysis, 2009
The notion of simple compact quantum group is introduced. As non-trivial (noncommutative and noncocommutative) examples, the following families of compact quantum groups are shown to be simple: (a) The universal quantum groups $B_u(Q)$ for $Q \in GL(n, {\mathbb C})$ satisfying $Q \bar{Q} = \pm I_n$, $n \geq 2$; (b) The quantum automorphism groups $A_ ...
openaire   +2 more sources

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