Results 81 to 90 of about 1,011,623 (291)

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Simultaneous multiple-emitter fitting for single molecule super-resolution imaging

Biomedical Optics Express, 2011
Single molecule localization based super-resolution imaging techniques require repeated localization of many single emitters. We describe a method that uses the maximum likelihood estimator to localize multiple emitters simultaneously within a single, two-dimensional fitting sub-region, yielding an order of magnitude improvement in the tolerance of the
Huang, Fang, Schwartz, Samantha L., Byars, Jason M., Lidke, Keith A.   +3 more
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Orthogonal distance fitting of precision free form surfaces based on l1 norm [PDF]

, 2008
Precision free-form surfaces are widely used in advanced optical and mechanical devices. In order to evaluate the form quality of a free-form surface, it is required to fit the measurement data with the design template and compare the relative deviation ...
Zhang, Xiangchao, Jiang, Xiang, Scott, Paul J.   +2 more
core   +4 more sources

Logarithmic sensitivity ratio elucidates thermal transport physics in multivariate thermoreflectance experiments

Fundamental Research
We mathematically derived a sensitivity-based method that identifies the thermal transport physics and parameters suitable for multivariate nonlinear fits in a frequency-domain thermoreflectance (FDTR) experiment.
Jing Tu, Md Azimul Haque, Derya Baran, Wee-Liat Ong   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Improving Plane Fitting Accuracy with Rigorous Error Models of Structured Light-Based RGB-D Sensors

Remote Sensing, 2020
Plane fitting is a fundamental operation for point cloud data processing. Most existing methods for point cloud plane fitting have been developed based on high-quality Lidar data giving equal weight to the point cloud data.
Yaxin Li, Wenbin Li, Walid Darwish, Shengjun Tang, Yuling Hu, Wu Chen   +5 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Determination of Electrochemical Parameters for Electrode Reaction of La(III)/La(0) Redox Couple in a High-Temperature Molten Salt Solution

International Journal of Electrochemical Science, 2014
The electrochemical parameters on the electrodeposition of lanthanum ions in the LiCl-KCl eutectic salts were determined by linear sweep voltammetry and curve fitting program.
Tack-Jin Kim, Eun Hee Kim, Do-Hee Ahn, Kwang-Rak Kim, Seung-Woo Paek, Yongju Jung   +5 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source