Results 11 to 20 of about 1,198,587 (324)

Single-cell RNA-sequencing in asthma research

Frontiers in Immunology, 2022
Asthma is a complex and heterogeneous disease with multicellular involvement, and knowledge gaps remain in our understanding of the pathogenesis of asthma. Efforts are still being made to investigate the immune pathogenesis of asthma in order to identify
Weifeng Tang, Weifeng Tang, Mihui Li, Mihui Li, Fangzhou Teng, Fangzhou Teng, Jie Cui, Jie Cui, Jingcheng Dong, Jingcheng Dong, Wenqian Wang, Wenqian Wang   +11 more
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Clinical Perspectives of Single-Cell RNA Sequencing [PDF]

Biomolecules, 2021
The ability of single-cell genomics to resolve cellular heterogeneity is highly appreciated in cancer and is being exploited for precision medicine. In the recent decade, we have witnessed the incorporation of cancer genomics into the clinical decision ...
Nayoung Kim, Hye Hyeon Eum, Hae-Ock Lee
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Single-cell RNA sequencing in orthopedic research

Bone Research, 2023
Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid ...
Tao Wang, Ling Wang, Liping Zhang, Yubin Long, Yingze Zhang, Zhiyong Hou   +5 more
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Single-Cell RNA Sequencing in Parkinson’s Disease [PDF]

Biomedicines, 2021
Single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) technologies have enhanced the understanding of the molecular pathogenesis of neurodegenerative disorders, including Parkinson’s disease (PD).
Shi-Xun Ma, Su Bin Lim
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Single-cell RNA-sequencing of the brain [PDF]

Clinical and Translational Medicine, 2017
Single-cell RNA-sequencing (scRNA-seq) is revolutionizing our understanding of the genomic, transcriptomic and epigenomic landscapes of cells within organs.
Raquel Cuevas-Diaz Duran, Haichao Wei, Jia Qian Wu   +2 more
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scDA: Single cell discriminant analysis for single-cell RNA sequencing data

Computational and Structural Biotechnology Journal, 2021
Single-cell RNA-sequencing (scRNA-seq) techniques provide unprecedented opportunities to investigate phenotypic and molecular heterogeneity in complex biological systems.
Qianqian Shi, Xinxing Li, Qirui Peng, Chuanchao Zhang, Luonan Chen   +4 more
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From bulk, single-cell to spatial RNA sequencing [PDF]

International Journal of Oral Science, 2021
RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing.
Xinmin Li, Cun-Yu Wang
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SMARTer single cell total RNA sequencing [PDF]

Nucleic Acids Research, 2019
Abstract Single cell RNA sequencing methods have been increasingly used to understand cellular heterogeneity. Nevertheless, most of these methods suffer from one or more limitations, such as focusing only on polyadenylated RNA, sequencing of only the 3′ end of the transcript, an exuberant fraction of reads mapping to ribosomal RNA ...
Karen Verboom, Celine Everaert, Nathalie Bolduc, Kenneth J Livak, Nurten Yigit, Dries Rombaut, Jasper Anckaert, Simon Lee, Morten T Venø, Jørgen Kjems, Frank Speleman, Pieter Mestdagh, Jo Vandesompele   +12 more
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Single-cell RNA sequencing in juvenile idiopathic arthritis

Genes and Diseases
Juvenile idiopathic arthritis (JIA) is one of the most common chronic inflammatory rheumatic diseases in children, with onset before age 16 and lasting for more than 6 weeks.
Xiwen Luo, Xuemei Tang
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Single-cell RNA sequencing v1 [PDF]

, 2021
Single-cell RNA sequencing (scRNA-seq) allows for transcriptional profiling of individual cells within a heterogenous sample. This protocol describes a method for performing scRNA-seq using handpicked pancreatic islets from organ donors.
Klaus H. Kaestner Lab, Suzanne Shapira
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