Results 41 to 50 of about 1,214,396 (338)

Single Cell RNA Sequencing in Atherosclerosis Research [PDF]

Circulation Research, 2020
Technological advances in characterizing molecular heterogeneity at the single cell level have ushered in a deeper understanding of the biological diversity of cells present in tissues including atherosclerotic plaques. New subsets of cells have been discovered among cell types previously considered homogenous. The commercial availability of systems to
Jesse W. Williams, Holger Winkels, Christopher P. Durant, Konstantin Zaitsev, Yanal Ghosheh, Klaus Ley   +5 more
openaire   +2 more sources

Delineating COVID-19 immunological features using single-cell RNA sequencing

The Innovation, 2022
Understanding the molecular mechanisms of coronavirus disease 2019 (COVID-19) pathogenesis and immune response is vital for developing therapies. Single-cell RNA sequencing has been applied to delineate the cellular heterogeneity of the host response ...
Wendao Liu, Johnathan Jia, Yulin Dai, Wenhao Chen, Guangsheng Pei, Qiheng Yan, Zhongming Zhao   +6 more
doaj   +1 more source

MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data

Genome Biology, 2015
Single-cell transcriptomics reveals gene expression heterogeneity but suffers from stochastic dropout and characteristic bimodal expression distributions in which expression is either strongly non-zero or non-detectable.
Greg Finak, A. McDavid, Masanao Yajima, Jingyuan Deng, V. Gersuk, A. Shalek, Chloe K. Slichter, Hannah W. Miller, M. McElrath, Martin Prlic, P. Linsley, R. Gottardo   +11 more
semanticscholar   +1 more source

Single Cell RNA Sequencing of Rare Immune Cell Populations

Frontiers in Immunology, 2018
Single-cell RNA sequencing (scRNA-Seq) is transforming our ability to characterize cells, particularly rare cells that are often overlooked in bulk population analytical approaches.
Akira Nguyen, Akira Nguyen, Weng Hua Khoo, Weng Hua Khoo, Imogen Moran, Imogen Moran, Peter I. Croucher, Peter I. Croucher, Tri Giang Phan, Tri Giang Phan   +9 more
doaj   +1 more source

Normalizing single-cell RNA sequencing data: challenges and opportunities [PDF]

, 2017
Single-cell transcriptomics is becoming an important component of the molecular biologist's toolkit. A critical step when analyzing data generated using this technology is normalization.
A Mortazavi, A Scialdone, A Zeisel, A-E Saliba, AA Kolodziejczyk, AA Kolodziejczyk, AA Pollen, AM Klein, Antonio Scialdone, AT Lun, B Ding, B Li, C Gawad, C Trapnell, CA Vallejos, CA Vallejos, Catalina A Vallejos, D Grün, D Risso, Davide Risso, E Pierson, E Shapiro, EZ Macosko, F Buettner, F Tang, G Finak, GK Smyth, J Lovén, JH Bullard, John C Marioni, JT Leek, L Haghverdi, L Jiang, M Goolam, M-A Dillies, MD Robinson, O Stegle, P Brennecke, PV Kharchenko, R Bacher, S Anders, S Islam, S Islam, S Katayama, SA Hardwick, SA Munro, Sandrine Dudoit, X Qiu   +47 more
core   +2 more sources

A molecular cell atlas of the human lung from single cell RNA sequencing

Nature, 2019
Although single-cell RNA sequencing studies have begun to provide compendia of cell expression profiles1–9, it has been difficult to systematically identify and localize all molecular cell types in individual organs to create a full molecular cell atlas.
K. Travaglini, Ahmad N. Nabhan, L. Penland, Rahul Sinha, A. Gillich, R. Sit, Stephen Chang, Stephanie D. Conley, Y. Mori, J. Seita, G. Berry, J. Shrager, Ross J. Metzger, Christin S Kuo, N. Neff, I. Weissman, S. Quake, M. Krasnow   +17 more
semanticscholar   +1 more source

Single-Cell RNA-Sequencing in Glioma

Current Oncology Reports, 2018
In this review, we seek to summarize the literature concerning the use of single-cell RNA-sequencing for CNS gliomas.Single-cell analysis has revealed complex tumor heterogeneity, subpopulations of proliferating stem-like cells and expanded our view of tumor microenvironment influence in the disease process.
Eli, Johnson, Katherine L, Dickerson, Ian D, Connolly, Melanie, Hayden Gephart   +3 more
openaire   +3 more sources

SoupX removes ambient RNA contamination from droplet-based single-cell RNA sequencing data

bioRxiv, 2018
Droplet based single cell RNA sequence analyses assume all acquired RNAs are endogenous to cells. However, any cell free RNAs contained within the input solution are also captured by these assays. This sequencing of cell free RNA constitutes a background
Matthew D. Young, S. Behjati
semanticscholar   +1 more source

Single-cell RNA sequencing v2

, 2021
Single-cell RNA sequencing (scRNA-seq) allows for transcriptional profiling of individual cells within a heterogenous sample. This protocol describes a method for performing scRNA-seq using handpicked pancreatic islets from organ donors.
Klaus H. Kaestner Lab, Suzanne Shapira
openaire   +1 more source

The Application of Single-Cell RNA Sequencing in Vaccinology

Journal of Immunology Research, 2020
Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher
Andrés Noé, Tamsin N. Cargill, Carolyn M. Nielsen, Andrew J. C. Russell, Eleanor Barnes   +4 more
doaj   +1 more source