Results 51 to 60 of about 1,214,396 (338)

Microglia Heterogeneity in Alzheimer’s Disease: Insights From Single-Cell Technologies

Frontiers in Synaptic Neuroscience, 2021
Microglia are resident immune cells in the central nervous system and play critical roles in brain immunity, development, and homeostasis. The pathology of Alzheimer’s disease (AD) triggers activation of microglia.
Hansen Wang
doaj   +1 more source

PanglaoDB: a web server for exploration of mouse and human single-cell RNA sequencing data

Database J. Biol. Databases Curation, 2019
Single-cell RNA sequencing is an increasingly used method to measure gene expression at the single cell level and build cell-type atlases of tissues. Hundreds of single-cell sequencing datasets have already been published. However, studies are frequently
Oscar Franzén, L. Gan, J. Björkegren
semanticscholar   +1 more source

Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma

Nature Communications, 2020
Advanced metastatic cancer poses utmost clinical challenges and may present molecular and cellular features distinct from an early-stage cancer. Herein, we present single-cell transcriptome profiling of metastatic lung adenocarcinoma, the most prevalent ...
N. Kim, H. Kim, K. Lee, Yourae Hong, J. Cho, J. Choi, Jung-Il Lee, Y. Suh, B. Ku, Hye Hyeon Eum, Soyean Choi, Yoon-La Choi, Je-Gun Joung, W. Park, H. Jung, Jong-Mu Sun, Se-Hoon Lee, J. Ahn, Keunchil Park, M. Ahn, Hae-Ock Lee   +20 more
semanticscholar   +1 more source

Pumping the brakes on RNA velocity by understanding and interpreting RNA velocity estimates

Genome Biology, 2023
Background RNA velocity analysis of single cells offers the potential to predict temporal dynamics from gene expression. In many systems, RNA velocity has been observed to produce a vector field that qualitatively reflects known features of the system ...
Shijie C. Zheng, Genevieve Stein-O’Brien, Leandros Boukas, Loyal A. Goff, Kasper D. Hansen   +4 more
doaj   +1 more source

SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology [PDF]

, 2018
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Abdelmoez Mahmoud N., Iida Kei, Kotera Hidetoshi, Nishikii Hidekazu, Oguchi Yusuke, Santiago Juan G., Shintaku Hirofumi, Uemura Sotaro, Yokokawa Ryuji, 錦井 秀和   +9 more
core   +2 more sources

Single cell RNA sequencing research in maternal fetal interface

Frontiers in Cell and Developmental Biology, 2023
The maternal-fetal interface is an essential environment for embryonic growth and development, and a successful pregnancy depends on the dynamic balance of the microenvironment at the maternal-fetal interface.
Qian Chen, Qian Chen, Dan Shan, Dan Shan, Yupei Xie, Yupei Xie, Xingrong Luo, Xingrong Luo, Yuxia Wu, Yuxia Wu, Qiuhe Chen, Qiuhe Chen, Ruihong Dong, Ruihong Dong, Yayi Hu, Yayi Hu, Yayi Hu   +16 more
doaj   +1 more source

A benchmark of batch-effect correction methods for single-cell RNA sequencing data

Genome Biology, 2020
Large-scale single-cell transcriptomic datasets generated using different technologies contain batch-specific systematic variations that present a challenge to batch-effect removal and data integration.
Hoa T M Tran, Kok Siong Ang, Marion Chevrier, Xiaomeng Zhang, N. Lee, Michelle Goh, Jinmiao Chen   +6 more
semanticscholar   +1 more source

Single‐Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche‐Associated Heterogeneity

Immunity, 2018
Lauren B. Rodda, Erick Lu, Mariko L. Bennett, Caroline L. Sokol, Xiaoming Wang, S. Luther, B. Barres, A. Luster, Chun Jimmie Ye, J. Cyster   +9 more
semanticscholar   +3 more sources

Experimental design for single-cell RNA sequencing [PDF]

Briefings in Functional Genomics, 2017
Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet ...
Baran-Gale, Jeanette, Chandra, Tamir, Kirschner, Kristina   +2 more
openaire   +4 more sources

Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis

Science Advances, 2019
Single-cell RNA sequencing provides new insights into pathologic epithelial and mesenchymal remodeling in the human lung. Pulmonary fibrosis (PF) is a form of chronic lung disease characterized by pathologic epithelial remodeling and accumulation of ...
A. C. Habermann, A. Gutierrez, L. Bui, Stephanie L. Yahn, N. Winters, C. Calvi, L. Peter, M. Chung, Chase J. Taylor, C. Jetter, Latha Raju, J. Roberson, G. Ding, L. Wood, J. Sucre, B. Richmond, A. Serezani, Wyatt J. McDonnell, Simon Mallal, M. Bacchetta, J. Loyd, C. Shaver, L. Ware, R. Bremner, R. Walia, T. Blackwell, N. Banovich, JA Kropski   +27 more
semanticscholar   +1 more source