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Introduction to Single‐Cell RNA Sequencing
Current Protocols in Molecular Biology, 2018AbstractDuring the last decade, high‐throughput sequencing methods have revolutionized the entire field of biology. The opportunity to study entire transcriptomes in great detail using RNA sequencing (RNA‐seq) has fueled many important discoveries and is now a routine method in biomedical research. However, RNA‐seq is typically performed in “bulk,” and
Thale Kristin, Olsen, Ninib, Baryawno
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Single-cell RNA sequencing: An overview for the ophthalmologist
Seminars in Ophthalmology, 2021Understanding the molecular composition of pathogenic tissues is a critical step in understanding the pathophysiology of disease and designing therapeutics. First described in 2009, single cell RNA sequencing (scRNAseq) is a methodology whereby thousands of cells are simultaneously isolated into individual micro-environments that can be altered ...
Elizabeth J. Rossin +2 more
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Single‐Cell RNA Sequencing in Hematological Diseases
PROTEOMICS, 2020Abstract Hematological diseases, including leukemia, lymphoma, and multiple myeloma, are characterized by high heterogeneity with diverse cellular subpopulations. Single‐cell RNA sequencing (scRNA‐seq), a transformational technology, provides deep insights into cell‐to‐cell variation in tumor and microenvironment, allows high ...
Yue Zhu +4 more
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Single-cell sequencing of the small-RNA transcriptome
Nature Biotechnology, 2016Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells.
Omid R Faridani +5 more
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Single-Cell RNA Sequencing with Drop-Seq
2019Drop-Seq is a low-cost, high-throughput platform to profile thousands of cells by encapsualting them into individual droplets. Uniquely barcoded mRNA capture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized
Josephine, Bageritz, Gianmarco, Raddi
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Single-Cell RNA Sequencing of Glioblastoma Cells
2018Single-cell RNA sequencing (sc-RNASeq) is a recently developed technique used to evaluate the transcriptome of individual cells. As opposed to conventional RNASeq in which entire populations are sequenced in bulk, sc-RNASeq can be beneficial when trying to better understand gene expression patterns in markedly heterogeneous populations of cells or when
Rajeev, Sen +5 more
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Preparation of single cells from tumors for single-cell RNA sequencing
2020Intratumoral heterogeneity of cancer cells and tumor-infiltrating immune cells is increasingly being viewed as a key factor driving tumor progression and response to therapy. Over the past several years, technological advances have created powerful tools to analyze the tumor microenvironment on a single-cell level, including mass cytometry and single ...
Yonina, Bykov +2 more
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Depletion of Ribosomal RNA Sequences from Single‐Cell RNA‐Sequencing Library
Current Protocols in Molecular Biology, 2016AbstractRecent advances in single‐cell RNA sequencing technologies have revealed high heterogeneity of gene expression profiles in individual cells. However, most current single‐cell RNA‐seq methods use oligo‐dT priming in the reverse transcription steps and detect only polyA‐positive for more accuracy, since there are also polyA‐positive non‐coding ...
Nan, Fang, Rumeysa, Akinci-Tolun
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Single-cell RNA-Sequencing in Neuroscience
Neuroforum, 2019Summary Technical innovations in the last decade have allowed to sequence transcriptomes of single cells. Single-cell RNA-sequencing (scRNA-seq) has since then opened the window to a deeper understanding of cellular identity and is becoming a widely used method in molecular biology.
Simone Mayer +3 more
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High-throughput single-cell RNA sequencing
Trends in Plant Science, 2022Tom Denyer, Marja C.P. Timmermans
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