Results 21 to 30 of about 980,999 (298)

Single-cell RNA Sequencing in Immunology

Current Genomics, 2020
The complex immune system is involved in multiple pathological processes. Single-cell RNA sequencing (scRNA-seq) is able to analyze complex cell mixtures correct to a single cell and single molecule, thus is qualified to analyze immune reactions in several diseases.
Yirui Cao, Yue Qiu, Guowei Tu, Cheng Yang   +3 more
openaire   +3 more sources

SPsimSeq : semi-parametric simulation of bulk and single-cell RNA-sequencing data [PDF]

, 2020
SPsimSeq is a semi-parametric simulation method to generate bulk and single-cell RNA-sequencing data. It is designed to simulate gene expression data with maximal retention of the characteristics of real data.
Assefa, Alemu Takele, Thas, Olivier, Vandesompele, Jo   +2 more
core   +1 more source

Delineating COVID-19 immunological features using single-cell RNA sequencing

The Innovation, 2022
Understanding the molecular mechanisms of coronavirus disease 2019 (COVID-19) pathogenesis and immune response is vital for developing therapies. Single-cell RNA sequencing has been applied to delineate the cellular heterogeneity of the host response ...
Wendao Liu, Johnathan Jia, Yulin Dai, Wenhao Chen, Guangsheng Pei, Qiheng Yan, Zhongming Zhao   +6 more
doaj   +1 more source

Single-cell RNA sequencing identifies distinct mouse medial ganglionic eminence cell types. [PDF]

, 2017
Many subtypes of cortical interneurons (CINs) are found in adult mouse cortices, but the mechanism generating their diversity remains elusive. We performed single-cell RNA sequencing on the mouse embryonic medial ganglionic eminence (MGE), the major ...
Chen, Ying-Jiun J, Durinck, Steffen, Friedman, Brad A, Ha, Connie, Liu, Jinfeng, Modrusan, Zora, Rubenstein, John L, Seshagiri, Somasekar   +7 more
core   +1 more source

Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy [PDF]

, 2020
Chimeric antigen receptor (CAR) T-cell therapy has produced remarkable anti-tumor responses in patients with B-cell malignancies. However, clonal kinetics and transcriptional programs that regulate the fate of CAR-T cells after infusion remain poorly ...
Adair, Jennifer E., DeBerg, Hannah A., Enstrom, Mark R., Gersuk, Vivian, Gottardo, Raphaël, Hanafi, Laïla-Aïcha, Hawkins, Reed, Kiem, Hans-Peter, Linsley, Peter S., Maloney, David G., Pande, Dnyanada, Riddell, Stanley R., Sheih, Alyssa, Turtle, Cameron J., Voillet, Valentin, Wohlfahrt, Martin E., Yajima, Masanao   +16 more
core   +1 more source

Single-cell RNA sequencing of human kidney [PDF]

Scientific Data, 2020
AbstractA comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells. To investigate the classification and transcriptomic information of the human kidney, we rapidly obtained a single-cell suspension ...
Liao, Jinling, Yu, Zhenyuan, Chen, Yang, Bao, Mengying, Zou, Chunlin, Zhang, Haiying, Liu, Deyun, Li, Tianyu, Zhang, Qingyun, Li, Jiaping, Cheng, Jiwen, Mo, Zengnan   +11 more
openaire   +2 more sources

Single Cell RNA Sequencing in Atherosclerosis Research [PDF]

Circulation Research, 2020
Technological advances in characterizing molecular heterogeneity at the single cell level have ushered in a deeper understanding of the biological diversity of cells present in tissues including atherosclerotic plaques. New subsets of cells have been discovered among cell types previously considered homogenous. The commercial availability of systems to
Jesse W. Williams, Holger Winkels, Christopher P. Durant, Konstantin Zaitsev, Yanal Ghosheh, Klaus Ley   +5 more
openaire   +2 more sources

Single Cell RNA Sequencing of Rare Immune Cell Populations

Frontiers in Immunology, 2018
Single-cell RNA sequencing (scRNA-Seq) is transforming our ability to characterize cells, particularly rare cells that are often overlooked in bulk population analytical approaches.
Akira Nguyen, Akira Nguyen, Weng Hua Khoo, Weng Hua Khoo, Imogen Moran, Imogen Moran, Peter I. Croucher, Peter I. Croucher, Tri Giang Phan, Tri Giang Phan   +9 more
doaj   +1 more source

Single-Cell RNA-Sequencing in Glioma

Current Oncology Reports, 2018
In this review, we seek to summarize the literature concerning the use of single-cell RNA-sequencing for CNS gliomas.Single-cell analysis has revealed complex tumor heterogeneity, subpopulations of proliferating stem-like cells and expanded our view of tumor microenvironment influence in the disease process.
Eli, Johnson, Katherine L, Dickerson, Ian D, Connolly, Melanie, Hayden Gephart   +3 more
openaire   +3 more sources

Normalizing single-cell RNA sequencing data: challenges and opportunities [PDF]

, 2017
Single-cell transcriptomics is becoming an important component of the molecular biologist's toolkit. A critical step when analyzing data generated using this technology is normalization.
A Mortazavi, A Scialdone, A Zeisel, A-E Saliba, AA Kolodziejczyk, AA Kolodziejczyk, AA Pollen, AM Klein, Antonio Scialdone, AT Lun, B Ding, B Li, C Gawad, C Trapnell, CA Vallejos, CA Vallejos, Catalina A Vallejos, D Grün, D Risso, Davide Risso, E Pierson, E Shapiro, EZ Macosko, F Buettner, F Tang, G Finak, GK Smyth, J Lovén, JH Bullard, John C Marioni, JT Leek, L Haghverdi, L Jiang, M Goolam, M-A Dillies, MD Robinson, O Stegle, P Brennecke, PV Kharchenko, R Bacher, S Anders, S Islam, S Islam, S Katayama, SA Hardwick, SA Munro, Sandrine Dudoit, X Qiu   +47 more
core   +2 more sources