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Technology in Cancer Research & Treatment, 2022 Introduction: The application of single-cell RNA sequencing to delineate tissue heterogeneity and complexity has become increasingly popular. Given its tremendous resolution and high-dimensional capacity for in-depth investigation, single-cell RNA ...
GS Chuwdhury MPhil +2 more
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Computational and Structural Biotechnology Journal, 2023 Obesity affects the function of multiple organs/tissues including the exocrine organ salivary glands. However, the effects of obesity on transcriptomes and cell compositions in the salivary glands have yet been studied by bulk RNA-sequencing and single ...
Heping Huang +5 more
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Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity. [PDF]
, 2018 Breast cancer arises from breast epithelial cells that acquire genetic alterations leading to subsequent loss of tissue homeostasis. Several distinct epithelial subpopulations have been proposed, but complete understanding of the spectrum of ...
Blake, Kerrigan +16 more
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Sample Multiplexing for Retinal Single-Cell RNA-Sequencing [PDF]
iScienceSummaryRare cell populations can be challenging to characterize using microfluidic single-cell RNA sequencing (scRNA-seq) platforms. Typically, the population of interest must be enriched and pooled from multiple biological specimens for efficient collection.
Justin Ma +8 more
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On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments [PDF]
, 2020 Background: In gene expression studies, RNA sample pooling is sometimes considered because of budget constraints or lack of sufficient input material. Using microarray technology, RNA sample pooling strategies have been reported to optimize both the cost
Assefa, Alemu Takele +2 more
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Revolutionizing immunology with single-cell RNA sequencing [PDF]
Cellular & Molecular Immunology, 2019 The immune system is composed of a complex hierarchy of cell types that protect the organism against disease and maintain homeostasis. Identifying heterogeneity of immune cells is the key to understanding the immune system. Advanced single-cell RNA sequencing (scRNA-seq) technologies are revolutionizing our ability to study immunology.
Haide, Chen, Fang, Ye, Guoji, Guo
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Molecular recording of mammalian embryogenesis. [PDF]
, 2019 Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. This field is particularly challenging in mammals, owing to the indeterminate relationship between self-renewal and differentiation, variation in progenitor
Adamson, Britt +13 more
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Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
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Targeting individual cells by barcode in pooled sequence libraries [PDF]
, 2019 Transcriptional profiling of thousands of single cells in parallel by RNA-seq is now routine. However, due to reliance on pooled library preparation, targeting analysis to particular cells of interest is difficult.
Blainey, Paul C +3 more
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Continual learning approaches for single cell RNA sequencing data
Scientific Reports, 2023 Single-cell RNA sequencing data is among the most interesting and impactful data of today and the sizes of the available datasets are increasing drastically.
Gorkem Saygili, Busra OzgodeYigin
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