Results 51 to 60 of about 1,215,495 (340)

Experimental design for single-cell RNA sequencing [PDF]

Briefings in Functional Genomics, 2017
Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet ...
Baran-Gale, Jeanette, Chandra, Tamir, Kirschner, Kristina   +2 more
openaire   +4 more sources

Intracerebral Hemorrhage and Ischemic Stroke of Different Etiologies Have Distinct Alternatively Spliced mRNA Profiles in the Blood: a Pilot RNA-seq Study. [PDF]

, 2015
Whole transcriptome studies have used 3'-biased expression microarrays to study genes regulated in the blood of stroke patients. However, alternatively spliced messenger RNA isoforms have not been investigated for ischemic stroke or intracerebral ...
Ander, Bradley P, Dykstra-Aiello, Cheryl, Ho, Carolyn, Hull, Heather, Jickling, Glen C, Liu, DaZhi, Orantia, Miles, Stamova, Boryana, Zhan, Xinhua   +8 more
core   +2 more sources

EmptyDrops: distinguishing cells from empty droplets in droplet-based single-cell RNA sequencing data

Genome Biology, 2019
Droplet-based single-cell RNA sequencing protocols have dramatically increased the throughput of single-cell transcriptomics studies. A key computational challenge when processing these data is to distinguish libraries for real cells from empty droplets.
A. Lun, Samantha J. Riesenfeld, T. Andrews, T. Dao, T. Gomes, J. Marioni   +5 more
semanticscholar   +1 more source

Molecular recording of mammalian embryogenesis. [PDF]

, 2019
Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. This field is particularly challenging in mammals, owing to the indeterminate relationship between self-renewal and differentiation, variation in progenitor
Adamson, Britt, Chan, Michelle M, Grosswendt, Stefanie, Jones, Matthew G, Jost, Marco, Khodaverdian, Alex, Kretzmer, Helene, Meissner, Alexander, Norman, Thomas M, Quinn, Jeffrey J, Smith, Zachary D, Weissman, Jonathan S, Yang, Dian, Yosef, Nir   +13 more
core   +2 more sources

Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy [PDF]

, 2020
Chimeric antigen receptor (CAR) T-cell therapy has produced remarkable anti-tumor responses in patients with B-cell malignancies. However, clonal kinetics and transcriptional programs that regulate the fate of CAR-T cells after infusion remain poorly ...
Adair, Jennifer E., DeBerg, Hannah A., Enstrom, Mark R., Gersuk, Vivian, Gottardo, Raphaël, Hanafi, Laïla-Aïcha, Hawkins, Reed, Kiem, Hans-Peter, Linsley, Peter S., Maloney, David G., Pande, Dnyanada, Riddell, Stanley R., Sheih, Alyssa, Turtle, Cameron J., Voillet, Valentin, Wohlfahrt, Martin E., Yajima, Masanao   +16 more
core   +1 more source

Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations

Nature Communications, 2018
The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment.
S. Macparland, Jeffrey C. Liu, Xuezhong Ma, Brendan T. Innes, A. Bartczak, B. K. Gage, J. Manuel, Nicholas Khuu, J. Echeverri, I. Linares, Rahul Gupta, Michael L Cheng, Lewis Y. Liu, Damra Camat, Sai W. Chung, Rebecca K Seliga, Zigong Shao, Elizabeth Lee, Shinichiro Ogawa, Mina Ogawa, M. Wilson, Jason E. Fish, M. Selzner, A. Ghanekar, D. Grant, P. Greig, G. Sapisochin, N. Selzner, N. Winegarden, O. Adeyi, G. Keller, Gary D Bader, I. McGilvray   +32 more
semanticscholar   +1 more source

scAnalyzeR: A Comprehensive Software Package With Graphical User Interface for Single-Cell RNA Sequencing Analysis and its Application on Liver Cancer

Technology in Cancer Research & Treatment, 2022
Introduction: The application of single-cell RNA sequencing to delineate tissue heterogeneity and complexity has become increasingly popular. Given its tremendous resolution and high-dimensional capacity for in-depth investigation, single-cell RNA ...
GS Chuwdhury MPhil, Irene Oi-Lin Ng MD, PhD, Daniel Wai-Hung Ho PhD   +2 more
doaj   +1 more source

Linked optical and gene expression profiling of single cells at high-throughput. [PDF]

, 2020
Single-cell RNA sequencing has emerged as a powerful tool for characterizing cells, but not all phenotypes of interest can be observed through changes in gene expression.
Abate, Adam R, Chang, Kai-Chun, Gartner, Zev J, Liu, Leqian, Siltanen, Christian A, Zhang, Jesse Q   +5 more
core   +1 more source

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources

Sample Multiplexing for Retinal Single-Cell RNA-Sequencing [PDF]

iScience
SummaryRare cell populations can be challenging to characterize using microfluidic single-cell RNA sequencing (scRNA-seq) platforms. Typically, the population of interest must be enriched and pooled from multiple biological specimens for efficient collection.
Justin Ma, Ting Kuan Chu, Maria Polo Prieto, Yong Park, Yumei Li, Rui Chen, Graeme Mardon, Benjamin J Frankfort, Nicholas M Tran   +8 more
openaire   +4 more sources