Results 101 to 110 of about 5,402,175 (290)
Ultra‐narrow green multi‐resonance thermally activated delayed fluorescence emitters have been developed by linear π‐extension strategy using tetracyclic naphtho[2,3‐b]benzofuran unit. The resulting phosphorescence‐sensitized OLEDs achieve a maximum external quantum efficiency of 37.7% in the bottom‐emitting architecture, while the complementary top ...
Eo Jin Jeon +7 more
wiley +1 more source
ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement
Fluorescence lifetime imaging microscopy (FLIM)-based Förster resonance energy transfer (FRET) measurement (FLIM-FRET) is one of the powerful methods for imaging of intracellular protein activities such as protein–protein interactions and conformational ...
Hideji Murakoshi, Akihiro C. E. Shibata
doaj +1 more source
Single Molecule FRET Analysis of DNA Binding Proteins
The complex binding dynamics between DNA and proteins are often obscured by ensemble averaging effects in conventional biochemical experiments. Single-molecule fluorescence methods are powerful tools to investigate DNA-protein interaction dynamics in real time. In this chapter, we focus on using single-molecule Förster Resonance Energy Transfer (smFRET)
Chaurasiya, K.R., Dame, R.T.
openaire +4 more sources
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal +21 more
wiley +1 more source
Time-resolved FRET -based approach for antibody detection - a new serodiagnostic concept.
Förster resonance energy transfer (FRET) is a phenomenon widely utilized in biomedical research of macromolecular interactions. In FRET energy is transferred between two fluorophores, the donor and the acceptor.
Satu Saraheimo +8 more
doaj +1 more source
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz +9 more
wiley +1 more source
A Bayesian Approach to Single-Photon Single-Molecule FRET data
: Single molecule FRET experiments are important for studying processes that happen on the molecular scale. By using pulsed illumination and collecting single photons, it is possible to use information gained from the fluorescence lifetime of the ...
core
Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells. [PDF]
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Yury Prokazov +20 more
core +2 more sources
RecA filament sliding on DNA facilitates homology search
During homologous recombination, RecA forms a helical filament on a single stranded (ss) DNA that searches for a homologous double stranded (ds) DNA and catalyzes the exchange of complementary base pairs to form a new heteroduplex.
Kaushik Ragunathan +2 more
doaj +1 more source
Analysis of Complex Single-Molecule FRET Time Trajectories [PDF]
Single-molecule methods have given researchers the ability to investigate the structural dynamics of biomolecules at unprecedented resolution and sensitivity. One of the preferred methods of studying single biomolecules is single-molecule fluorescence resonance energy transfer (smFRET).
Mario, Blanco, Nils G, Walter
openaire +2 more sources

