Results 131 to 140 of about 5,402,175 (290)

Single-molecule FRET assay for RAD51 WT- and RAD51 G151D- filament formation and RPA-RAD51 interaction.

, 2016
A. Illustration of single-molecule RAD51-ssDNA filament assay with partial DNA duplex containing a 30—nucleotide tail. Upon RAD51 filament formation, there is a transition from high FRET (DNA-only) to low FRET (RAD51-bound). B. Histograms display a clear
Ryan B. Jensen (2937867), Carolyn G. Marsden (119929), Eli Rothenberg (2149852), Jennifer Zagelbaum (2937864), Joann B. Sweasy (31229), Scott W. Morrical (423051), Susan S. Wallace (16874)   +6 more
core   +1 more source

Sustainable Poly(Lactic Acid)/Graphene Oxide Bioelectronic Platform for Neurotransmitters Sensing and Tunable Stimulation of Astrocytes

Advanced Materials, EarlyView.
This work introduces sustainable PLA/graphene oxide bioelectronic interfaces featuring electrodes with tunable conductivity and strong electrocatalytic performance. These platforms were used to implement a new method for tuning astrocyte Ca2+ signaling and for the efficient detection of key biomarkers.
Alessandra Scidà, Giulia Cazzador, Roberta Fabbri, Matteo Calosi, Alessandro Silvestri, Andrea Candini, Fabiola Liscio, Franco Corticelli, Aikaterini Konstantoulaki, Andrea D'Iorio, Marco Caprini, Vincenzo Palermo, Monica Bertoldo, Valentina Benfenati, Chiara Zanardi, Emanuele Treossi   +15 more
wiley   +1 more source

Temperature-cycle single-molecule FRET microscopy on polyprolines

, 2011
Accessing the microsecond dynamics of a single fluorescent molecule in real time is difficult because molecular fluorescence rates usually limit the time resolution to milliseconds.
Yuan, H, Yuan, Haifeng, Michel Orrit, Ted Xia, Orrit, M, Haifeng Yuan, Benjamin Schuler, Xia, Ted, Schuler, Benjamin, Schuler, B, Orrit, Michel, Xia, T   +11 more
core   +1 more source

Confinement‐Induced Donnan Potential Enables Sealed Hydrovoltaic Power From Microliter Water

Advanced Materials, EarlyView.
An ultrathin MXene/cellulose nanofiber (CNF) nanochannel‐based hydrovoltaic generator produces sustained direct current (DC) from a single microliter droplet under sealed operation. Asymmetric nanochannel confinement establishes a persistent ion gradient and a confinement‐induced Donnan potential, harvesting the interfacial free energy released upon ...
Sangyun Na, Geonyoung Jung, Yoojin Chang, Yun Goo Ro, Cheolhong Park, Jeonghee Yeom, Jinyoung Kim, Jeeyoon Kim, Hyejin Lee, Hyeji Oh, Hyunhyub Ko   +10 more
wiley   +1 more source

High precision FRET studies reveal reversible transitions in nucleosomes between microseconds and minutes

Nature Communications, 2018
Nucleosomes compact the genome and regulate access to specific DNA sequences. Here the authors employ single-molecule FRET studies to characterize nucleosome dynamics at different salt concentrations and dissect nucleosome disassembly into elementary ...
Alexander Gansen, Suren Felekyan, Ralf Kühnemuth, Kathrin Lehmann, Katalin Tóth, Claus A. M. Seidel, Jörg Langowski   +6 more
doaj   +1 more source

Electropolymerized Aptasensor for Femtogram‐Level Cytokine Detection

Advanced Materials, EarlyView.
Electropolymerization of 3‐amino‐ʟ‐tyrosine creates ultrathin, smooth, and chemically robust pALT nanofilms with accessible carboxyl functionalities for direct aptamer immobilization. Integrated with fiber‐optic SPR and OECT platforms, these interfaces enable IL‐6 detection down to 100 fg mL−1 and provide a versatile route to bioelectronic sensing ...
Jiyao Yu, Renan Colucci, Rachel X. Shi, Qi Lu, Tommaso Marchesi D'Alvise, Ulrike Kraft, Christopher V. Synatschke, Tanja Weil   +7 more
wiley   +1 more source

Esterified Cholesterol Conjugates Enable LNP‐Mediated mRNA Delivery to the Blood–Brain Barrier Following Intravenous Administration

Advanced Materials, EarlyView.
A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian, Xu Wang, Alexis Pacheco Benitez, Erick Guerrero, Yaou Duan, Jingwen Wei, Lukas Farbiak, Daniel J. Siegwart   +7 more
wiley   +1 more source

Photobleaching Pathways in Single-Molecule FRET Experiments

, 2016
To acquire accurate structural and dynamical information on complex biomolecular machines using single-molecule fluorescence resonance energy transfer (sm-FRET), a large flux of donor and acceptor photons is needed.
Xiangxu Kong (2490220), Eyal Nir (1868404), Kambiz Hamadani (2495653), Shimon Weiss (1290570)   +3 more
core   +1 more source

Redox‐Confined Interfacial Polymerization Enables Mechanically Robust and Self‐Adhesive Nanofilms

Advanced Materials Interfaces, EarlyView.
Redox‐confined polymerization of a simple thiol monomer at a water‐organic interface generates robust freestanding films with nanoscale thickness. The resulting disulfide‐crosslinked nanofilms combine high stiffness with intrinsic adhesion to soft substrates, enabling conformal contact with artificial skin without additional functionalization.
Luana Gazzato, Vivek Manish, Giorgio Speranza, Maria F. Pantano, Marco Frasconi   +4 more
wiley   +1 more source

Extrusion Bioprinting for Wound Healing: Innovations in Functionalized Bioinks and Bioprinting Technology

Advanced Materials Technologies, EarlyView.
Extrusion‐based bioprinting (EBB) has emerged as a versatile biofabrication platform capable of precisely depositing bioinks composed of biomaterials, cells, and bioactive agents to generate patient‐specific, biomimetic skin constructs. This paper presents a state‐of‐the‐art and forward‐looking overview of EBB for wound healing, encompassing printing ...
Hien‐Phuong Le, Anh Tuan Trong Tran, Ammara Waheed, Tran Thanh Tung, Dusan Losic   +4 more
wiley   +1 more source