Results 171 to 180 of about 5,805,397 (279)

Single-molecule FRET and tracking of transfected biomolecules in living cells. [PDF]

Biophys J
Anandamurugan A, Eidloth A, Frank V, Wortmann P, Schrangl L, Lan C, Schütz GJ, Hugel T.   +7 more
europepmc   +1 more source

Single-molecule FRET observes opposing effects of urea and TMAO on structurally similar meso- and thermophilic riboswitch RNAs. [PDF]

Nucleic Acids Res, 2023
Hou Q, Chatterjee S, Lund PE, Suddala KC, Walter NG.   +4 more
europepmc   +1 more source

A Versatile DNA‐Encoded Library Platform for the Discovery of Highly Cooperative Chemical Inducers of Proximity

Advanced Science, EarlyView.
We present a versatile DNA‐encoded library (DEL) platform designed for rapid library synthesis and subsequent screening for chemical inducers of proximity (CIPs) across various presenter proteins of choice. Applying this approach, we discovered a novel molecular glue‐like compound mediating CRBN‐BRD9 ternary complex formation with remarkable ...
Bingqi Tong, Sunny A. Tang, Yifan Deng, Zhihan Nan, Alison X. Gao, Gregory A. Michaud, Simone Bonazzi, Frédéric Berst, Frédéric J. Zécri, Shuang Liu, William J. Gibson, Stuart L. Schreiber   +11 more
wiley   +1 more source

Correlating membrane-protein dynamics with function: Integrating bioinformatics, molecular dynamics, and single-molecule FRET. [PDF]

Protein Sci
Higinbotham HR, Arbour CA, Imperiali B.
europepmc   +1 more source

Temporal Trajectories of the Tau Aggregate Interactome Reveal Stage‐Specific Vulnerabilities in Alzheimer's Disease

Advanced Science, EarlyView.
Tau populations with different aggregation states associate with distinct sets of proteins in the Alzheimer's disease brain. Proteomic discovery combined with single‐molecule analysis in neurons reveals dynamic associations involving proteostasis, metabolism and RNA biology.
Dorothea Böken, Paula Beltran Lobo, Yunzhao Wu, Lyla A. Rowe, Emre Fertan, Cara L. Croft, Maria Jimenez‐Sanchez, Dezerae Cox, David Klenerman   +8 more
wiley   +1 more source

Three-color single-molecule fluorescence resonance energy transfer to study macromolecular dynamics. [PDF]

Curr Opin Struct Biol
Gao J, Yuan B, Mondal S, Basak R, Lee TH.   +4 more
europepmc   +1 more source

Can DyeCycling break the photobleaching limit in single-molecule FRET? [PDF]

Nano Res, 2022
Vermeer B, Schmid S.
europepmc   +1 more source

Co‐activation of Selective Nicotinic Acetylcholine Receptor Subtypes Is Required for Neuroprotection against Alzheimer's Disease

Advanced Science, EarlyView.
ABSTRACT Beta‐amyloid peptide (Aβ)‐induced suppression of hippocampal GABAergic interneuron activity drives hyperexcitability, amyloid pathology, and cognitive impairment in Alzheimer's disease (AD), suggesting that enhancing hippocampal inhibition may be protective.
Rahmi Lee, Gayeon Kim, Ellison R. Black, Seonil Kim   +3 more
wiley   +1 more source

In-cell single-molecule FRET measurement of cytosolic RAF proteins to investigate the structural states and kinetics among them. [PDF]

Front Mol Biosci
Okamoto K, Sako Y.
europepmc   +1 more source

Tracking CDK1 Dynamics With a Novel Biosensor Mouse and Clinical Cohorts to Predict CDK4/6 Inhibitor Sensitivity in ER+ Breast Cancer

Advanced Science, EarlyView.
Cyclin‐dependent kinase (CDK) 1 activity is a dynamic marker of treatment response in estrogen receptor positive breast cancer. Patient cohorts reveal CDK1 activity increases during progression to invasive disease, where high expression correlates with poor survival. Using a novel Cdk1 biosensor mouse alongside in vitro models, we demonstrate that CDK1
Sarah Alexandrou, Kristine J. Fernandez, Min Li Huang, Ewan K. A. Millar, Claire Vennin, Leila Eshraghi, Tan Dat Timothy Lam, Victoria Lee, Nyah C. Brooks, Janett Stoehr, Cristian Guaman Mendez, Douglas Strathdee, David Stevenson, David Herrmann, Sandra O'Toole, Paul Timpson, Max Nobis, C. Elizabeth Caldon   +17 more
wiley   +1 more source