Results 71 to 80 of about 62,512 (285)
Single Molecule FRET Characterization of Large Ribozyme Folding [PDF]
, 2012 A procedure to investigate the folding of group II intron by single molecule Fluorescence Resonance Energy Transfer (smFRET) using total internal reflection fluorescence microscopy (TIRFM) is described in this chapter. Using our previous studies on the folding and dynamics of a large ribozyme in the presence of metal ions (i.e., Mg(2+) and Ca(2+)) and ...
Cardo, L +3 more
openaire +2 more sources
Frontiers in Molecular Biosciences, 2021 In addition to the canonical double helix form, DNA is known to be extrapolated into several other secondary structural patterns involving themselves in inter- and intramolecular type hydrogen bonding. The secondary structures of nucleic acids go through
Debolina Bandyopadhyay +3 more
doaj +1 more source
, 2013 FoF1-ATP synthase catalyzes the synthesis of adenosine triphosphate (ATP). The F1 portion can be stripped from the membrane-embedded Fo portion of the enzyme.
Boersch, Michael +4 more
core +1 more source
Identification of a 3-Alkylpyridinium Compound from the Red Sea Sponge Amphimedon chloros with In Vitro Inhibitory Activity against the West Nile Virus NS3 Protease. [PDF]
, 2018 Viruses are underrepresented as targets in pharmacological screening efforts, given the difficulties of devising suitable cell-based and biochemical assays.
Duggan, Brendan M +8 more
core +3 more sources
Laser‐Induced Graphene from Waste Almond Shells
Advanced Functional Materials, EarlyView.Almond shells, an abundant agricultural by‐product, are repurposed to create a fully bioderived almond shell/chitosan composite (ASC) degradable in soil. ASC is converted into laser‐induced graphene (LIG) by laser scribing and proposed as a substrate for transient electronics.
Yulia Steksova +9 more
wiley +1 more source
Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET
CHIMIA, 2019 Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible.
Fabio D. Steffen +3 more
doaj +1 more source
eLife, 2020 Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for studying the structure and dynamics of biomolecules. The development of high throughput methodologies and the growth of commercial instrumentation have outpaced the ...
Johannes Thomsen +8 more
doaj +1 more source
Time-resolved burst variance analysis
Biophysical Reports, 2023 Quantifying biomolecular dynamics has become a major task of single-molecule fluorescence spectroscopy methods. In single-molecule Förster resonance energy transfer (smFRET), kinetic information is extracted from the stream of photons emitted by attached
Ivan Terterov +3 more
doaj +1 more source
Advanced Functional Materials, EarlyView.This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti +3 more
wiley +1 more source
ABCE1 Controls Ribosome Recycling by an Asymmetric Dynamic Conformational Equilibrium
Cell Reports, 2019 Summary: The twin-ATPase ABCE1 has a vital function in mRNA translation by recycling terminated or stalled ribosomes. As for other functionally distinct ATP-binding cassette (ABC) proteins, the mechanochemical coupling of ATP hydrolysis to conformational
Giorgos Gouridis +7 more
doaj +1 more source