Results 51 to 60 of about 5,805,397 (279)
The smfBox: an open-source platform for single-molecule FRET [PDF]
Nature Communications, 2019 Abstract Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non ...
Benjamin Ambrose +10 more
openaire +5 more sources
The Development of Instrumentation to Increase the Accessibility and Throughput of Single-Molecule FRET [PDF]
, 2022 Current drug discovery approaches use high-throughput ensemble methods combined with large libraries of small molecules to measure and quantify the drug-ligand interactions.
George, Dylan
core +2 more sources
Resolving dynamics and function of transient states in single enzyme molecules
Nature Communications, 2020 T4 Lysozyme (T4L) is a model protein whose structure is extensively studied. Here the authors combine single-molecule and ensemble FRET measurements, FRET-positioning and screening and EPR spectroscopy to study the structural dynamics of T4L and describe
Hugo Sanabria +11 more
doaj +1 more source
Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2
Nature Communications, 2022 Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.
Sarah Willkomm +6 more
doaj +1 more source
eLife, 2021 Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample ...
Eitan Lerner +45 more
doaj +1 more source
Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α-Fig. 2def
, 2017 <p>smTIRF-FRET Data for Fig 2, for "Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α"</p ...
Bryan, Louise C. +19 more
core +1 more source
Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α-Fig. 1df
, 2017 <p>smTIRF-FRET Data for Fig 1, for "Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α"</p ...
Bryan, Louise C. +19 more
core +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source