Results 51 to 60 of about 5,402,175 (290)
Single-molecule nano-optics at low temperature [PDF]
, 2006 The work presented in this thesis involves experiments on single molecules at low temperature. At low temperature, many disturbing temperature activated processes are frozen out, resulting in extremely sharp zero-phonon lines in the fluorescence ex ...
Butter, Jacqueline Yvonne Paula
core +1 more source
Fast three-color single-molecule FRET using statistical inference
Nature Communications, 2020 Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity.
Janghyun Yoo +4 more
doaj +1 more source
Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates [PDF]
, 2013 This article was made OA through RCUK block grant funds.Flap endonuclease 1 (Fen1) is a highly conserved structure-specific nuclease that catalyses a specific incision to remove 5′ flaps in double-stranded DNA substrates.
Brenlla, Alfonso +9 more
core +1 more source
eLife, 2021 Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample ...
Eitan Lerner +45 more
doaj +1 more source
The smfBox: an open-source platform for single-molecule FRET [PDF]
Nature Communications, 2019 Abstract Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non ...
Benjamin Ambrose +10 more
openaire +5 more sources
Resolving dynamics and function of transient states in single enzyme molecules
Nature Communications, 2020 T4 Lysozyme (T4L) is a model protein whose structure is extensively studied. Here the authors combine single-molecule and ensemble FRET measurements, FRET-positioning and screening and EPR spectroscopy to study the structural dynamics of T4L and describe
Hugo Sanabria +11 more
doaj +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Single-Molecule FRET in Living Bacteria [PDF]
Biophysical Journal, 2012 Most single-molecule FRET experiments are performed in vitro, using tightly controlled conditions and well-defined concentrations of a limited number of interacting components. However, in order to understand biological mechanisms as they occur in vivo while simultaneously taking advantage of the extra information provided by single-molecule detection,
Crawford, R, Torella, J, Kapanidis, A
openaire +2 more sources
Biophysical approaches for studying viral entry
FEBS Letters, EarlyView.Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source