Results 31 to 40 of about 5,402,175 (290)

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach [PDF]

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina   +13 more
core   +1 more source

Dual-probe RNA FRET-FISH in Yeast

Bio-Protocol, 2018
mRNA Fluorescence In Situ Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can also be used ...
Gable Wadsworth, Rasesh Parikh, Harold Kim   +2 more
doaj   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard, Richard Ankerhold, Drummen, Gregor P. C., Gregor P. C. Drummen, Hellen C. Ishikawa-Ankerhold, Ishikawa-Ankerhold, Hellen C.   +5 more
core   +1 more source

Structural heterogeneity and quantitative FRET efficiency distributions of polyprolines through a hybrid atomistic simulation and Monte Carlo approach.

PLoS ONE, 2011
Förster Resonance Energy Transfer (FRET) experiments probe molecular distances via distance dependent energy transfer from an excited donor dye to an acceptor dye.
Martin Hoefling, Nicola Lima, Dominik Haenni, Claus A M Seidel, Benjamin Schuler, Helmut Grubmüller   +5 more
doaj   +1 more source

Protein folding studied by single-molecule FRET [PDF]

Current Opinion in Structural Biology, 2008
A complete understanding of a protein-folding mechanism requires description of the distribution of microscopic pathways that connect the folded and unfolded states. This distribution can, in principle, be described by computer simulations and theoretical models of protein folding, but is hidden in conventional experiments on large ensembles of ...
Schuler, B, Eaton, W A
openaire   +4 more sources

FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected Aptamers

, 2023
Iron-regulated surface determinant protein A (IsdA) is a key surface protein found in the foodborne bacteriaStaphylococcus aureus (S. aureus)which is known to be critical for bacterial survival and colonization. S.
Chamika Harshani Algama (16384871), Soma Dhakal, Chamika Harshani Algama, Godfred Sabbih (16384868), Soma Dhakal (156075), Kalani M. Wijesinghe (12270260), Rida Syed, Godfred Sabbih, Michael K. Danquah, Kalani M. Wijesinghe, Rida Syed (16384874), Michael K. Danquah (16384877)   +11 more
core   +1 more source

Studying DNA Looping by Single-Molecule FRET [PDF]

Journal of Visualized Experiments, 2014
Bending of double-stranded DNA (dsDNA) is associated with many important biological processes such as DNA-protein recognition and DNA packaging into nucleosomes. Thermodynamics of dsDNA bending has been studied by a method called cyclization which relies on DNA ligase to covalently join short sticky ends of a dsDNA.
Tung T, Le, Harold D, Kim
openaire   +2 more sources

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S., Laptenok, S., Visser, N.V., Hoek, A., van, Laptenok, S.P., Borst, J.W., Visser, A.J.W.G., van Hoek, A., Brochon, J.C.   +8 more
core   +1 more source

Impact of Small Molecules on Intermolecular G-Quadruplex Formation

Molecules, 2019
We performed single molecule studies to investigate the impact of several prominent small molecules (the oxazole telomestatin derivative L2H2-6OTD, pyridostatin, and Phen-DC3) on intermolecular G-quadruplex (i-GQ) formation between two guanine-rich DNA ...
Prabesh Gyawali, Keshav GC, Yue Ma, Sanjaya Abeysirigunawardena, Kazuo Nagasawa, Hamza Balci   +5 more
doaj   +1 more source

Fluorescence resonance energy transfer studies of protein interactions [PDF]

, 2008
This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
core   +2 more sources