Results 31 to 40 of about 5,805,397 (279)

Studying DNA Looping by Single-Molecule FRET [PDF]

Journal of Visualized Experiments, 2014
Bending of double-stranded DNA (dsDNA) is associated with many important biological processes such as DNA-protein recognition and DNA packaging into nucleosomes. Thermodynamics of dsDNA bending has been studied by a method called cyclization which relies on DNA ligase to covalently join short sticky ends of a dsDNA.
Tung T, Le, Harold D, Kim
openaire   +2 more sources

Dual-probe RNA FRET-FISH in Yeast

Bio-Protocol, 2018
mRNA Fluorescence In Situ Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can also be used ...
Gable Wadsworth, Rasesh Parikh, Harold Kim   +2 more
doaj   +1 more source

The many states of STIM1

eLife, 2021
Harnessing single-molecule FRET illuminates the structural changes necessary for a protein to fine-tune the influx of calcium when reserves inside a cell run low.
Marc Fahrner, Christoph Romanin
doaj   +1 more source

Structural heterogeneity and quantitative FRET efficiency distributions of polyprolines through a hybrid atomistic simulation and Monte Carlo approach.

PLoS ONE, 2011
Förster Resonance Energy Transfer (FRET) experiments probe molecular distances via distance dependent energy transfer from an excited donor dye to an acceptor dye.
Martin Hoefling, Nicola Lima, Dominik Haenni, Claus A M Seidel, Benjamin Schuler, Helmut Grubmüller   +5 more
doaj   +1 more source

Photobleaching Pathways in Single-Molecule FRET Experiments [PDF]

Journal of the American Chemical Society, 2007
To acquire accurate structural and dynamical information on complex biomolecular machines using single-molecule fluorescence resonance energy transfer (sm-FRET), a large flux of donor and acceptor photons is needed. To achieve such fluxes, one may use higher laser excitation intensity; however, this induces increased rates of photobleaching.
Xiangxu, Kong, Eyal, Nir, Kambiz, Hamadani, Shimon, Weiss   +3 more
openaire   +2 more sources

FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected Aptamers

, 2023
Iron-regulated surface determinant protein A (IsdA) is a key surface protein found in the foodborne bacteriaStaphylococcus aureus (S. aureus)which is known to be critical for bacterial survival and colonization. S.
Chamika Harshani Algama (16384871), Soma Dhakal, Chamika Harshani Algama, Godfred Sabbih (16384868), Soma Dhakal (156075), Kalani M. Wijesinghe (12270260), Rida Syed, Godfred Sabbih, Michael K. Danquah, Kalani M. Wijesinghe, Rida Syed (16384874), Michael K. Danquah (16384877)   +11 more
core   +1 more source

Impact of Small Molecules on Intermolecular G-Quadruplex Formation

Molecules, 2019
We performed single molecule studies to investigate the impact of several prominent small molecules (the oxazole telomestatin derivative L2H2-6OTD, pyridostatin, and Phen-DC3) on intermolecular G-quadruplex (i-GQ) formation between two guanine-rich DNA ...
Prabesh Gyawali, Keshav GC, Yue Ma, Sanjaya Abeysirigunawardena, Kazuo Nagasawa, Hamza Balci   +5 more
doaj   +1 more source

Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles

Biophysical Reports, 2023
Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations. At the single-
Quinten Coucke, Nagma Parveen, Guillermo Solís Fernández, Chen Qian, Johan Hofkens, Zeger Debyser, Jelle Hendrix   +6 more
doaj   +1 more source

FRET Efficiency Distributions of Multistate Single Molecules [PDF]

The Journal of Physical Chemistry B, 2010
A simple analytic theory is developed to describe FRET efficiency histograms constructed from a photon trajectory generated by a molecule with multiple conformational states. The histograms are approximated by a sum of Gaussians with the parameters explicitly determined by the FRET efficiencies of the states and the rates of the transitions between the
Irina V, Gopich, Attila, Szabo
openaire   +2 more sources

Single-Step FRET-Based Detection of Femtomoles DNA

Sensors, 2019
Sensitive detection of nucleic acids and identification of single nucleotide polymorphism (SNP) is crucial in diagnosis of genetic diseases. Many strategies have been developed for detection and analysis of DNA, including fluorescence, electrical ...
Kumar Sapkota, Anisa Kaur, Anoja Megalathan, Caleb Donkoh-Moore, Soma Dhakal   +4 more
doaj   +1 more source