Results 41 to 50 of about 5,805,397 (279)
Hexameric helicase G40P unwinds DNA in single base pair steps
eLife, 2019 Most replicative helicases are hexameric, ring-shaped motor proteins that translocate on and unwind DNA. Despite extensive biochemical and structural investigations, how their translocation activity is utilized chemo-mechanically in DNA unwinding is ...
Michael Schlierf +3 more
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Future Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy [PDF]
Biophysical Journal, 2016 Förster (or fluorescence) resonance energy transfer (FRET) is a nonradiative energy transfer process between two fluorophores located in close proximity to each other. To date, a variety of biosensors based on the principle of FRET have been developed to monitor the activity of kinases, proteases, GTPases or lipid concentration in living cells.
Eishu, Hirata, Etsuko, Kiyokawa
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Single-molecule FRET studies in live bacteria [PDF]
, 2016 Single-molecule fluorescence and single-molecule Förster resonance energy transfer (FRET) have proven enormously successful in understanding molecular and cellular processes over the last two decades.
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Computational refinement of spectroscopic FRET measurements
Data in Brief, 2017 This article supplies raw data related to a research article entitled “Joint refinement of FRET measurements using spectroscopic and computational tools” (Kyrychenko et al., 2017) [1], in which we demonstrate the use of molecular dynamics simulations to ...
Alexander Kyrychenko +3 more
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ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling [PDF]
Nature Methods, 2019 ABSTRACT Single-molecule Förster resonance energy transfer (smFRET) has become a versatile and widespread method to probe nanoscale conformation and dynamics. However, current experimental protocols often resort to molecule immobilization for long observation times and rarely approach the resolution limit of FRET-based nanoscale ...
Hugh Wilson, Quan Wang
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Don’t Be Fooled by Randomness: Valid p-Values for Single Molecule Microscopy
Frontiers in Bioinformatics, 2022 The human mind shows extraordinary capability at recognizing patterns, while at the same time tending to underestimate the natural scope of random processes. Taken together, this easily misleads researchers in judging whether the observed characteristics
Magdalena C. Schneider +1 more
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Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard +5 more
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Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach [PDF]
, 2015 People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo +17 more
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Fast three-color single-molecule FRET using statistical inference
Nature Communications, 2020 Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity.
Janghyun Yoo +4 more
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ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling
, 2021 Raw Data related to the manuscript: "ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling" Contains the following files: ReadMe.txt - a quick introduction to the upload ABEL-FRET-data-format-exampleCode.docx - a detailed ...
Wilson, Hugh, Wang, Quan
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