Results 41 to 50 of about 5,402,175 (290)
Single-Step FRET-Based Detection of Femtomoles DNA
Sensors, 2019 Sensitive detection of nucleic acids and identification of single nucleotide polymorphism (SNP) is crucial in diagnosis of genetic diseases. Many strategies have been developed for detection and analysis of DNA, including fluorescence, electrical ...
Kumar Sapkota +4 more
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Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles
Biophysical Reports, 2023 Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations. At the single-
Quinten Coucke +6 more
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FRET Efficiency Distributions of Multistate Single Molecules [PDF]
The Journal of Physical Chemistry B, 2010 A simple analytic theory is developed to describe FRET efficiency histograms constructed from a photon trajectory generated by a molecule with multiple conformational states. The histograms are approximated by a sum of Gaussians with the parameters explicitly determined by the FRET efficiencies of the states and the rates of the transitions between the
Irina V, Gopich, Attila, Szabo
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songuno1/single-molecule-assay: Single-molecule assay analysis
, 2023 Single-molecule assay analysis tool for "Transcriptional pause extension benefits the stand-by rather than catch-up Rho-dependent termination"
Eunho Song
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ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling [PDF]
Nature Methods, 2019 ABSTRACT Single-molecule Förster resonance energy transfer (smFRET) has become a versatile and widespread method to probe nanoscale conformation and dynamics. However, current experimental protocols often resort to molecule immobilization for long observation times and rarely approach the resolution limit of FRET-based nanoscale ...
Hugh Wilson, Quan Wang
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Computational refinement of spectroscopic FRET measurements
Data in Brief, 2017 This article supplies raw data related to a research article entitled “Joint refinement of FRET measurements using spectroscopic and computational tools” (Kyrychenko et al., 2017) [1], in which we demonstrate the use of molecular dynamics simulations to ...
Alexander Kyrychenko +3 more
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Future Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy [PDF]
Biophysical Journal, 2016 Förster (or fluorescence) resonance energy transfer (FRET) is a nonradiative energy transfer process between two fluorophores located in close proximity to each other. To date, a variety of biosensors based on the principle of FRET have been developed to monitor the activity of kinases, proteases, GTPases or lipid concentration in living cells.
Eishu, Hirata, Etsuko, Kiyokawa
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Single-molecule FRET studies in live bacteria [PDF]
, 2016 Single-molecule fluorescence and single-molecule Förster resonance energy transfer (FRET) have proven enormously successful in understanding molecular and cellular processes over the last two decades.
Plochowietz, Anne, Anne Plochowietz
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Hexameric helicase G40P unwinds DNA in single base pair steps
eLife, 2019 Most replicative helicases are hexameric, ring-shaped motor proteins that translocate on and unwind DNA. Despite extensive biochemical and structural investigations, how their translocation activity is utilized chemo-mechanically in DNA unwinding is ...
Michael Schlierf +3 more
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Don’t Be Fooled by Randomness: Valid p-Values for Single Molecule Microscopy
Frontiers in Bioinformatics, 2022 The human mind shows extraordinary capability at recognizing patterns, while at the same time tending to underestimate the natural scope of random processes. Taken together, this easily misleads researchers in judging whether the observed characteristics
Magdalena C. Schneider +1 more
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