Results 11 to 20 of about 5,402,175 (290)
BOBA FRET: bootstrap-based analysis of single-molecule FRET data. [PDF]
PLoS ONE, 2013 Time-binned single-molecule Förster resonance energy transfer (smFRET) experiments with surface-tethered nucleic acids or proteins permit to follow folding and catalysis of single molecules in real-time. Due to the intrinsically low signal-to-noise ratio
Sebastian L B König +6 more
doaj +5 more sources
Single-Molecule FRET of Membrane Transport Proteins. [PDF]
Chembiochem, 2021 AbstractUncovering the structure and function of biomolecules is a fundamental goal in structural biology. Membrane‐embedded transport proteins are ubiquitous in all kingdoms of life. Despite structural flexibility, their mechanisms are typically studied by ensemble biochemical methods or by static high‐resolution structures, which complicate a ...
Bartels K +3 more
europepmc +6 more sources
Single-Molecule Three-Color FRET [PDF]
Biophysical Journal, 2004 Fluorescence resonance energy transfer (FRET) measured at the single-molecule level can reveal conformational changes of biomolecules and intermolecular interactions in physiologically relevant conditions. Thus far single-molecule FRET has been measured only between two fluorophores.
Hohng, Sungchul +2 more
openaire +4 more sources
Evaluation of FRET X for single-molecule protein fingerprinting [PDF]
iScience, 2021 Summary: Single-molecule protein identification is an unrealized concept with potentially ground-breaking applications in biological research. We propose a method called FRET X (Förster Resonance Energy Transfer via DNA eXchange) fingerprinting, in which
Carlos Victor de Lannoy +4 more
doaj +6 more sources
Closing the gap between single molecule and bulk FRET analysis of nucleosomes. [PDF]
PLoS ONE, 2013 Nucleosome structure and stability affect genetic accessibility by altering the local chromatin morphology. Recent FRET experiments on nucleosomes have given valuable insight into the structural transformations they can adopt.
Alexander Gansen +4 more
doaj +1 more source
eLife, 2022 Single-molecule fluorescence spectroscopy and molecular dynamics simulations illuminate the structure and dynamics of PSD-95, a protein involved in neural plasticity.
Ivan Maslov, Jelle Hendrix
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Signal Transduction Mechanisms Quantitatively Observed One Molecule at a Time
Frontiers in Physics, 2022 Improved single-molecule methods can largely increase our understanding of underlying molecular mechanism during cellular signal transduction. In contrast to conventional bulk methods, monitoring molecules one at a time can circumvent averaging effects ...
Pei Li +4 more
doaj +1 more source
On the statistical foundation of a recent single molecule FRET benchmark [PDF]
Nature CommunicationsAyush Saurabh +2 more
doaj +2 more sources
A practical guide to single-molecule FRET [PDF]
Nature Methods, 2008 Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by biophysicists. We provide a practical guide to using smFRET, focusing
Rahul, Roy, Sungchul, Hohng, Taekjip, Ha
openaire +2 more sources
Rapid quantification of miRNAs using dynamic FRET-FISH
Communications Biology, 2022 The high-speed single-molecule miRNA detection technique dynamic FRET-FISH shows increased detection speed and high target specificity, detecting a single miRNA in ten seconds.
Juyoung Kim +3 more
doaj +1 more source