The potential of fluorogenicity for single molecule FRET and DyeCycling [PDF]
QRB DiscoverySingle Molecule Förster Resonance Energy Transfer (smFRET) is a popular technique to directly observe biomolecular dynamics in real time, offering unique mechanistic insight into proteins, ribozymes, and so forth.
Srijayee Ghosh, Sonja Schmid
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Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation [PDF]
Nature Communications, 2023 Biomolecular condensates formed via phase separation of proteins and nucleic acids are thought to be associated with a wide range of cellular functions and dysfunctions.
Ashish Joshi +6 more
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Single-Molecule FRET: A Tool to Characterize DNA Nanostructures [PDF]
Frontiers in Molecular Biosciences, 2022 DNA nanostructures often involve temporally evolving spatial features. Tracking these temporal behaviors in real time requires sophisticated experimental methods with sufficiently high spatial and temporal resolution.
Nibedita Pal
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Biosensors, 2020 Single-molecule fluorescence energy transfer (FRET) detection has become a key technique to monitor intra- and intermolecular distance changes in biological processes.
Heyjin Son +4 more
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Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET [PDF]
Communications Biology, 2023 Single-molecule FRET experiments with human A2A adenosine receptor in its apo and agonist-bound states in lipid nanodiscs provide insights into its conformational dynamics and activation.
Ivan Maslov +18 more
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Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET [PDF]
Cell Reports: MethodsSummary: Single-molecule Förster resonance energy transfer (smFRET) provides nanometer-scale snapshots of biomolecular conformational dynamics, but extracting reliable dynamic information from diffusion-based measurements requires a careful choice of ...
Joel A. Crossley
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Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET [PDF]
BiomoleculesRiboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg2+ and their ligands playing pivotal roles in this process.
Yanyan Xue +4 more
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Single-molecule FRET–based approach for protein-targeted drug discovery [PDF]
Molecules and Cells: Many therapeutic drugs target various proteins involved in diverse biological processes. Among these proteins, type II topoisomerases are critical targets for anticancer and antibacterial chemotherapies, yet the action mechanisms of many type II ...
Yuyoung Kim +3 more
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Single-molecule three-color FRET with both negligible spectral overlap and long observation time. [PDF]
PLoS ONE, 2010 Full understanding of complex biological interactions frequently requires multi-color detection capability in doing single-molecule fluorescence resonance energy transfer (FRET) experiments.
Sanghwa Lee, Jinwoo Lee, Sungchul Hohng
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BOBA FRET: bootstrap-based analysis of single-molecule FRET data. [PDF]
PLoS ONE, 2013 Time-binned single-molecule Förster resonance energy transfer (smFRET) experiments with surface-tethered nucleic acids or proteins permit to follow folding and catalysis of single molecules in real-time. Due to the intrinsically low signal-to-noise ratio
Sebastian L B König +6 more
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