Results 71 to 80 of about 5,402,175 (290)

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Monitoring multiple distances within a single molecule using switchable FRET [PDF]

, 2010
Uphoff S, Holden SJ, Le Reste L, et al. Monitoring multiple distances within a single molecule using switchable FRET. NATURE METHODS. 2010;7(10):831-836.The analysis of structure and dynamics of biomolecules is important for understanding their function.
Mike Heilemann, Stephan Uphoff, Sebastian van de Linde, Uphoff, S, Ludovic Le Reste, Holden, Seamus J., van de Linde, Sebastian, Kapanidis, Achillefs N., Seamus J Holden, Holden, Seamus, Holden, SJ, Achillefs N Kapanidis, Kapanidis, AN, Periz, J, Le Reste, Ludovic, Heilemann, M, Periz, Javier, van de Linde, S, Javier Periz, Uphoff, Stephan, Heilemann, Mike, Le Reste, L   +21 more
core   +1 more source

ABCE1 Controls Ribosome Recycling by an Asymmetric Dynamic Conformational Equilibrium

Cell Reports, 2019
Summary: The twin-ATPase ABCE1 has a vital function in mRNA translation by recycling terminated or stalled ribosomes. As for other functionally distinct ATP-binding cassette (ABC) proteins, the mechanochemical coupling of ATP hydrolysis to conformational
Giorgos Gouridis, Bianca Hetzert, Kristin Kiosze-Becker, Marijn de Boer, Holger Heinemann, Elina Nürenberg-Goloub, Thorben Cordes, Robert Tampé   +7 more
doaj   +1 more source

Time-resolved burst variance analysis

Biophysical Reports, 2023
Quantifying biomolecular dynamics has become a major task of single-molecule fluorescence spectroscopy methods. In single-molecule Förster resonance energy transfer (smFRET), kinetic information is extracted from the stream of photons emitted by attached
Ivan Terterov, Daniel Nettels, Dmitrii E. Makarov, Hagen Hofmann   +3 more
doaj   +1 more source

Single-molecule FRET measures bends and kinks in DNA [PDF]

Proceedings of the National Academy of Sciences, 2008
We present advances in the use of single-molecule FRET measurements with flexibly linked dyes to derive full 3D structures of DNA constructs based on absolute distances. The resolution obtained by this single-molecule approach harbours the potential to study in detail also protein- or damage-induced DNA bending.
Wozniak, Anna K., Schröder, Gunnar F., Grubmüller, Helmut, Seidel, Claus A. M., Oesterhelt, Filipp   +4 more
openaire   +4 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Identification of binding mechanisms in single molecule-DNA complexes [PDF]

, 2003
Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario, Wilking, Sven David, Ros, Robert, Eckel, Rainer, Sven David Wilking, Dario Anselmetti, Robert Ros, Wilking, S.D., Sewald, Norbert ; https://orcid.org/, Ra Ros, Ros, Alexandra, Sewald, Norbert, Rainer Eckel, Anselmetti, Dario ; https://orcid.org/, Y Norbert Sewald   +14 more
core   +1 more source

ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling

, 2021
Raw Data related to the manuscript: "ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling" Contains the following files: ReadMe.txt - a quick introduction to the upload ABEL-FRET-data-format-exampleCode.docx - a detailed ...
Wilson, Hugh, Wang, Quan
core   +1 more source

Optimal Background Estimators in Single-Molecule FRET Microscopy [PDF]

Biophysical Journal, 2016
Single-molecule total internal reflection fluorescence (TIRF) microscopy constitutes an umbrella of powerful tools that facilitate direct observation of the biophysical properties, population heterogeneities, and interactions of single biomolecules without the need for ensemble synchronization.
Preus, Soren, Hildebrandt, Lasse L., Birkedal, Victoria   +2 more
openaire   +3 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source