Results 141 to 150 of about 1,316,697 (275)

Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack

Journal of Synchrotron Radiation
This work investigates the performance of the electrospray aerosol generator at the European X-ray Free Electron Laser (EuXFEL). This generator is, together with an aerodynamic lens stack that transports the particles into the X-ray interaction vacuum ...
Safi Rafie-Zinedine, Tej Varma Yenupuri, Lena Worbs, Filipe R. N. C. Maia, Michael Heymann, Joachim Schulz, Johan Bielecki   +6 more
doaj   +1 more source

Single-shot femtosecond x-ray diffraction from randomly oriented ellipsoidal nanoparticles

Physical Review Special Topics. Accelerators and Beams, 2010
Coherent diffractive imaging of single particles using the single-shot “diffract and destroy” approach with an x-ray free electron laser (FEL) was recently demonstrated.
M. J. Bogan, S. Boutet, A. Barty, W. H. Benner, M. Frank, L. Lomb, R. Shoeman, D. Starodub, M. M. Seibert, S. P. Hau-Riege, B. Woods, P. Decorwin-Martin, S. Bajt, J. Schulz, U. Rohner, B. Iwan, N. Timneanu, S. Marchesini, I. Schlichting, J. Hajdu, H. N. Chapman   +20 more
doaj   +1 more source

Introducing a frequency-tunable magnetic particle spectrometer

Current Directions in Biomedical Engineering, 2015
Image quality in the new imaging modality magnetic particle imaging (MPI) heavily relies on the quality of the magnetic nanoparticles in use. Therefore, it is crucial to understand the behaviour of such particles.
Behrends André, Graeser Matthias, Buzug Thorsten M.   +2 more
doaj   +1 more source

The effect of inhomogenities on single molecule imaging by hard XFEL pulses

, 2009
We study the local distortion of the atomic structure in small biological samples illuminated by x-ray free electron laser (XFEL) pulses. We concentrate on the effect of inhomogenities: heavy atoms in a light matrix and non-homogeneous spatial ...
Faigel, Gyula, Jurek, Zoltan
core   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy

International Journal of Nanomedicine, 2018
Abu S M Mohsin,1 Mariam B Salim2 1Centre for Micro-Photonics, Department of Physics, Swinburne University of Technology, Melbourne, VIC 3122, Australia; 2Electrical and Telecommunication Engineering, Swinburne University of Technology, Melbourne, VIC ...
Mohsin ASM, Salim MB
doaj  

Imaging Live Cells at the Nanometer-Scale with Single-Molecule Microscopy: Obstacles and Achievements in Experiment Optimization for Microbiology

Molecules, 2014
Single-molecule fluorescence microscopy enables biological investigations inside living cells to achieve millisecond- and nanometer-scale resolution. Although single-molecule-based methods are becoming increasingly accessible to non-experts, optimizing ...
Beth L. Haas, Jyl S. Matson, Victor J. DiRita, Julie S. Biteen   +3 more
doaj   +1 more source

Trends in Pixel Detectors: Tracking and Imaging

, 2004
For large scale applications, hybrid pixel detectors, in which sensor and read-out IC are separate entities, constitute the state of the art in pixel detector technology to date.
Wermes, Norbert
core   +2 more sources

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source