Results 71 to 80 of about 495,951 (309)

Computational study of diffraction image formation from XFEL irradiated single ribosome molecule

Scientific Reports
Single particle imaging at atomic resolution is perhaps one of the most desired goals for ultrafast X-ray science with X-ray free-electron lasers.
Michal Stransky, Juncheng E, Zoltan Jurek, Robin Santra, Richard Bean, Beata Ziaja, Adrian P. Mancuso   +6 more
doaj   +1 more source

4D Imaging and Diffraction Dynamics of Single-Particle Phase Transition in Heterogeneous Ensembles [PDF]

, 2014
In this Letter, we introduce conical-scanning dark-field imaging in four-dimensional (4D) ultrafast electron microscopy to visualize single-particle dynamics of a polycrystalline ensemble undergoing phase transitions.
Ahmed H. Zewail, Appavoo K., Appavoo K., Babkin E. V., Baik J. M., Balandin A. A., Barwick B., Baum P., Berglund C. N., Cao J., Cavalleri A., Cavalleri A., Cavalleri A., Chae B.-G., Chen J., Coy H., Dingley D. J., Dingley D. J., Duda J. C., Flannigan D. J., Fu D., Gavini A., Grinolds M. S., Hada M., Haihua Liu, Hawkes P. W., Jackson J. D., Jau Tang, Kana Kana J. B., Knoll M., Kwon O.-H., Kwon O.-H., Kwon O.-H., Kübler C., Leroux Ch., Liu H. H., Lobastov V. A., Lopez R., Lysenko S., Lysenko S., Morin F. J., Nair R. R., Narayan J., Norris P. M, Oh D.-W., Oh-Hoon Kwon, Okimura K., Park H. S., Pergament A., Picard S., Pierson H. O., Poulsen H. F., Prasher R., Schmidt S., Van der Veen R. M., Wang Z. Q., Williams D. B., Wu J. M., Yao T., Yurtsever A., Zeuner S., Zewail A. H., Zewail A. H.   +62 more
core   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Impact of ionization potential depression on single particle imaging

Physical Review Research
Ultraintense and ultrashort x-ray free-electron laser (XFEL) pulses promise single particle imaging (SPI) via enabling collection of diffraction data for nanocrystals, or nano-size nonperiodic objects before the destruction of the sample. Photoionization
Sourav Banerjee, Zoltan Jurek, Rui Jin, Sang-Kil Son, Robin Santra   +4 more
doaj   +1 more source

Fluorescent silica manoparticles with well-separated intensity distributions from batch reactions [PDF]

, 2018
Silica chemistry provides pathways to uniquely tunable nanoparticle platforms for biological imaging. It has been a long-standing problem to synthesize fluorescent silica nanoparticles (SNPs) in batch reactions with high and low fluorescence intensity ...
Andrievsky, Alexander, Aubert, Tangi, Kao, Teresa, Kohle, Ferdinand, Ma, Kai, Wiesner, Ulrich   +5 more
core   +2 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles

Frontiers in Physiology, 2014
Over the past decade, great developments in optical microscopy have made this technology increasingly compatible with biological studies. Fluorescence microscopy has especially contributed to investigating the dynamic behaviors of live specimens and can ...
Taro eIchimura, Takashi eJin, Takashi eJin, Takashi eJin, Hideaki eFujita, Hideaki eFujita, Hideo eHiguchi, Tomonobu M Watanabe, Tomonobu M Watanabe, Tomonobu M Watanabe   +9 more
doaj   +1 more source

Direct probing of single-molecule chemiluminescent reaction dynamics under catalytic conditions in solution

Nature Communications, 2023
Chemical reaction kinetics can be evaluated by probing dynamic changes of chemical substrates or physical phenomena accompanied during the reaction process.
Ziqing Zhang, Jinrun Dong, Yibo Yang, Yuan Zhou, Yuang Chen, Yang Xu, Jiandong Feng   +6 more
doaj   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source