Results 81 to 90 of about 173,038 (236)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

High-throughput in situ single particle X-ray imaging of dehydrating viral capsids

Light: Science & Applications
Single-stranded RNA viruses co-assemble their capsid with the genome, and variations in capsid structures can have significant functional relevance. In particular, viruses need to respond to a dehydrating environment to prevent genomic degradation and ...
Abhishek Mall, Anna Munke, Parichita Mazumder, Zhou Shen, Johan Bielecki, Juncheng E, Armando D. Estillore, Chan Kim, Romain Letrun, Jannik Lübke, Safi Rafie-Zinedine, Adam Round, Ekaterina Round, Michael Rütten, Amit K. Samanta, Abhisakh Sarma, Tokushi Sato, Florian Schulz, Carolin Seuring, Tamme Wollweber, Lena Worbs, Patrik Vagovic, Richard Bean, Adrian P. Mancuso, Ne-Te Duane Loh, Tobias Beck, Jochen Küpper, Filipe R.N.C. Maia, Henry N. Chapman, Kartik Ayyer   +29 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

A Maximum-Likelihood Approach to Single-Particle Image Refinement

Journal of Structural Biology, 1998
The alignment of single-particle images fails at low signal-to-noise ratios and small particle sizes, because noise produces false peaks in the cross-correlation function used for alignment. A maximum-likelihood approach to the two-dimensional alignment problem is described which allows the underlying structure to be estimated from large data sets of ...
openaire   +2 more sources

An ultrafast plenoptic-camera system for high-resolution 3D particle tracking in unsegmented scintillators

Nature Communications
Neutrino detectors, particle calorimeters and some dark matter detectors require dense and massive active materials. An extremely fine segmentation is desirable to achieve precise three-dimensional particle tracking.
Till Dieminger, Saúl Alonso-Monsalve, Christoph Alt, Claudio Bruschini, Noemi Bührer, Edoardo Charbon, Kodai Kaneyasu, Tim Weber, Matthew Franks, Davide Sgalaberna   +9 more
doaj   +1 more source

Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams

IUCrJ, 2018
Ultra-bright femtosecond X-ray pulses generated by X-ray free-electron lasers (XFELs) can be used to image high-resolution structures without the need for crystallization.
Max F. Hantke, Johan Bielecki, Olena Kulyk, Daniel Westphal, Daniel S. D. Larsson, Martin Svenda, Hemanth K. N. Reddy, Richard A. Kirian, Jakob Andreasson, Janos Hajdu, Filipe R. N. C. Maia   +10 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Highly sensitive volumetric single-molecule imaging

Nanophotonics
Volumetric subcellular imaging has long been essential for studying structures and dynamics in cells and tissues. However, due to limited imaging speed and depth of field, it has been challenging to perform live-cell imaging and single-particle tracking.
Wang Le-Mei, Kim Jiah, Han Kyu Young
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source