Results 1 to 10 of about 10,451,032 (401)
Single-Molecule Biodosimetry. [PDF]
Anal ChemInferring characteristics of radiation exposure using biological molecules is extremely challenging. Current methods, in particular, lack a clear connection between dose and molecular response. Here, we demonstrate that resistive–pulse nanopore sensors enable single–molecule biodosimetry by quantifying the frequency of double–strand DNA scissions ...
Lamontagne M +6 more
europepmc +3 more sources
Lanthanide Single-Molecule Magnets [PDF]
Chemical Reviews, 2013 Researchers demonstrate that lanthanide single-molecule magnets (SMM) have the potential for new technological applications. SMMs can be considered as molecular analogues of classical bulk ferromagnets, making it possible to develop them for applications involving the storage and processing of digital information.
Woodruff, Daniel N. +2 more
openaire +4 more sources
Single-molecule localization microscopy
Nature Reviews Methods Primers, 2021 Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular ...
M. Lelek +9 more
semanticscholar +1 more source
Proceedings of the National Academy of Sciences, 2001 At first an experimental challenge, the ability to conduct experiments with single molecules has been strongly connected with progress in experimental techniques and instrumentation. Scanning probe techniques, such as the scanning tunneling microscope or the atomic force microscope (AFM), use sharp tips in close proximity (10−9 m) to a sample to ...
T, Basché, S, Nie, J M, Fernandez
openaire +2 more sources
Single-Molecule Enzymology [PDF]
Journal of Biological Chemistry, 1999 Viewing a movie of an enzyme molecule made by molecular dynamics simulation, we see incredible details of molecular motions, be they changes of the conformation or actions during a chemical reaction. Molecular dynamics simulations have advanced our understanding of the dynamics of macromolecules in ways that would not be deducible from the static ...
X S, Xie, H P, Lu
openaire +2 more sources
Single‐molecule bioelectronics [PDF]
WIREs Nanomedicine and Nanobiotechnology, 2014 Experimental techniques that interface single biomolecules directly with microelectronic systems are increasingly being used in a wide range of powerful applications, from fundamental studies of biomolecules to ultra‐sensitive assays. In this study, we review several technologies that can perform electronic measurements of single molecules in solution:
Rosenstein, Jacob K. +2 more
openaire +3 more sources
Nature Communications, 2021 Ring ATPase translocases that operate on disordered substrates adopt lockwasher architectures and use a hand-over-hand mechanism. By challenging the dsDNA packaging motor of bacteriophage ϕ29 with hybrid and dsRNA, the authors propose that the motor ...
Juan P. Castillo +4 more
doaj +1 more source
Clinical Chemistry, 2011 The need for assay methods of sufficient sensitivity to determine the low concentrations of hormones present in body fluids led to the original development of immunoassays and analogous “binding” (or “ligand”) assays in the late 1950s and early 1960s. These methods depend on the use of a binding agent (also commonly referred to by other terms such as ...
Roger, Ekins, David, Kelso
openaire +2 more sources
An anionic ligand snap-locks a long-range interaction in a magnesium-folded riboswitch
Nature Communications, 2022 A single molecule fluorescence study revealed three dynamically interconverting conformations of the fluoride riboswitch from Bacillus cereus, where an anionic ligand snap-locks a docked conformation through a long-range interaction necessary for ...
Rajeev Yadav +3 more
doaj +1 more source
Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
Nature Communications, 2020 During eukaryotic chromosome replication cells utilize ring-shaped CMG helicase that separates the two strands of the DNA double helix. Here the authors reveal that CMG helicase activity is inhibited by duplex DNA engagement at the fork, which is ...
Hazal B. Kose +4 more
doaj +1 more source