Results 21 to 30 of about 10,451,032 (401)
Direct-laser writing for subnanometer focusing and single-molecule imaging
Nature Communications, 2022 Focus-locking improves localization precision in single-molecule microscopy, but fiducials are often deposited at random and provide limited 3D compensation.
Simao Coelho +4 more
doaj +1 more source
Single Molecule Nanometronome [PDF]
Nano Letters, 2006 We constructed a DNA-based nanomechanical device called the nanometronome. Our device is made by introducing complementary single-stranded overhangs at the two arms of the DNA four-way junction. The ticking rates of this stochastic metronome depend on ion concentrations and can be changed by a set of DNA-based switches to deactivate/reactivate the ...
Chittanon, Buranachai +2 more
openaire +2 more sources
Single-Molecule Reaction-Diffusion
Biophysical Journal, 2023 AbstractWe propose to capture reaction-diffusion on a molecule-by-molecule basis from the fastest acquirable timescale, namely individual photon arrivals. We illustrate our method on intrinsically disordered human proteins, the linker histone H1.0 as well as its chaperone prothymosinα, as these diffuse through an illuminated confocal spot and interact ...
Lance W.Q. Xu +3 more
openaire +3 more sources
eLife, 2022 Single molecule imaging has shown that part of actin disassembles within a few seconds after incorporation into the dendritic filament network in lamellipodia, suggestive of frequent destabilization near barbed ends.
Danielle Holz +5 more
doaj +1 more source
Single-Molecule Studies of Protein Folding with Optical Tweezers.
Annual Review of Biochemistry, 2020 Manipulation of individual molecules with optical tweezers provides a powerful means of interrogating the structure and folding of proteins. Mechanical force is not only a relevant quantity in cellular protein folding and function, but also a convenient ...
C. Bustamante +3 more
semanticscholar +1 more source
Nature Communications, 2016 In response to intracellular signals, bacterial translational riboswitches embedded in mRNAs can regulate gene expression through inhibition of translation initiation.
Arlie J. Rinaldi +3 more
doaj +1 more source
Phased diploid genome assembly with single-molecule real-time sequencing
Nature Methods, 2016 While genome assembly projects have been successful in many haploid and inbred species, the assembly of noninbred or rearranged heterozygous genomes remains a major challenge.
C. Chin +16 more
semanticscholar +1 more source
Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]
, 2013 The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore +54 more
core +1 more source
Magnetic hysteresis up to 80 kelvin in a dysprosium metallocene single-molecule magnet
Science, 2018 Breaking through the nitrogen ceiling Single-molecule magnets could prove useful in miniaturizing a wide variety of devices. However, their application has been severely hindered by the need to cool them to extremely low temperature using liquid helium ...
Fu-Sheng Guo +5 more
semanticscholar +1 more source
iScience, 2021 Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting.
Tim N. Baldering +8 more
doaj +1 more source