Protocol for High-Throughput Analysis of Sister-Chromatids Contacts [PDF]
STAR Protocols, 2020 Summary: Sister chromatid interactions are a key step to ensure the successful segregation of sister chromatids after replication. Our knowledge about this phenomenon is mostly based on microscopy approaches, which have some constraints such as ...
Elena Espinosa +2 more
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Checkpoint proteins come under scrutiny [PDF]
eLife, 2013 Details are emerging of the interactions between the kinetochore and various spindle checkpoint proteins that ensure that sister chromatids are equally divided between daughter cells during cell division.
Maria Mora-Santos, Jonathan BA Millar
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Splicing together sister chromatids. [PDF]
EMBO J, 2014 Splicing of pre‐mRNAs is a necessary step for expression of the majority of genes in higher eukaryotes, and its regulation through alternative splice site selection shapes their proteomes. Defects in multiple splicing factors result in aberrant mitotic progression, although the molecular basis for this observation has remained elusive. Recent papers in
Valcárcel J, Malumbres M.
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Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling [PDF]
Frontiers in Physiology, 2018 Despite studying the various molecular mechanisms of hepatocellular carcinoma (HCC), effective drugs and biomarkers in HCC therapy are still scarce. The present study was designed to investigate dysregulated pathways, novel biomarkers and therapeutic ...
Baozhen Sun +5 more
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Compaction and segregation of sister chromatids via active loop extrusion [PDF]
eLife, 2016 The mechanism by which chromatids and chromosomes are segregated during mitosis and meiosis is a major puzzle of biology and biophysics. Using polymer simulations of chromosome dynamics, we show that a single mechanism of loop extrusion by condensins can
Anton Goloborodko +3 more
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A requirement for STAG2 in replication fork progression creates a targetable synthetic lethality in cohesin-mutant cancers [PDF]
Nature Communications, 2019 Cohesin complex mediates cohesion of sister chromatids and DNA loop formation. Here the authors show another role of cohesin in replication fork progression, suggesting a potential therapeutic strategy for cohesin-mutant cancers.
Gourish Mondal +4 more
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A lack of coordination between sister-chromatids segregation and cytokinesis in the oocytes of B6.YTIR (XY) sex-reversed female mice [PDF]
Scientific Reports, 2017 The B6.YTIR (XY) mouse develops bilateral ovaries despite the expression of the testis-determining gene Sry during gonadal differentiation. We reported that the oocytes of the XY female are defective in their cytoplasm, resulting in a failure in the ...
Jia-Qiao Zhu +2 more
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Tethering sister centromeres to each other suggests the spindle checkpoint detects stretch within the kinetochore. [PDF]
PLoS Genetics, 2014 The spindle checkpoint ensures that newly born cells receive one copy of each chromosome by preventing chromosomes from segregating until they are all correctly attached to the spindle.
Natalie J Nannas, Andrew W Murray
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Sister Chromatid Cohesion [PDF]
Cold Spring Harbor Perspectives in Biology, 2012 During S phase, not only does DNA have to be replicated, but also newly synthesized DNA molecules have to be connected with each other. This sister chromatid cohesion is essential for the biorientation of chromosomes on the mitotic or meiotic spindle, and is thus an essential prerequisite for chromosome segregation.
Peters, J.-M. and Nishiyama, T.
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Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I [PDF]
Open Biology, 2021 Establishment of proper chromosome attachments to the spindle requires elimination of erroneous attachments, but the mechanism of this process is not fully understood.
Misuzu Wakiya +7 more
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