Results 111 to 120 of about 3,618,606 (252)

Site-Directed Mutagenesis of Green Fluorescent Protein

, 2017
Site-Directed Mutagenesis of Green Fluorescent ...
Huynh, Maria
core  

Auto‐Downregulation of the Florigen FT Production Prevents Precocious Flowering in Plants

Advanced Science, EarlyView.
This study shows that the leaf vein‐expressed FD complexes with the florigen FT to bind DNA motifs in FT promoter, which antagonizes CONSTANS‐mediated FT activation to feedback down‐regulate FT expression. This prevents an excessive induction of FT expression by inductive photoperiods, thereby precluding precocious transition to flowering in ...
Shu Tian, Xiao Luo, Bowen Cui, Yuehui He
wiley   +1 more source

Oligonucleotides used for general cloning and site-directed mutagenesis.

, 2015
Nucleotides altered by site directed mutagenesis are underlined.Oligonucleotides used for general cloning and site-directed mutagenesis.
Beate Sodeik (191751), Rudolf Bauerfeind (152093), Christina Funk (756280), Verena Raschbichler (756281), Anne Binz (309492), Melanie Ott (26926), Susanne M. Bailer (260258), Claus-Henning Nagel (309485)   +7 more
core   +1 more source

Mitochondrial SLC25A46 Rewires Fatty Acid Oxidation to Promote Cell Proliferation and Ferroptosis Evasion in Ovarian Cancer by Stabilizing CACT

Advanced Science, EarlyView.
Mitochondrial SLC25A46 is upregulated in ovarian cancer (OC) and correlates with poor prognosis. SLC25A46 stabilizes CACT to activate fatty acid oxidation, increasing ATP and NADPH production to drive cell proliferation and ferroptosis evasion. SLC25A46 silencing sensitizes OC cells to ferroptosis and carboplatin, offering a promising therapeutic ...
Yunge Gao, Jiatao Hao, Xiaohong Zhang, Meiting Long, Yujing Feng, Xiaoyue Liu, Xuejian Li, Shujuan Liu, Xin Guo, Xiaohui Lv   +9 more
wiley   +1 more source

Q5® Site-Directed Mutagenesis (E0552) v1

, 2014
This is the protocol for the Q5® Site-Directed Mutagenesis Kit without competent cells

core   +1 more source

Targeting GALNT7 Disrupts the TAZ O‐GalNAcylation Feedback Loop to Suppress Gallbladder Cancer Progression

Advanced Science, EarlyView.
Aberrant GALNT7‐mediated O‐GalNAcylation stabilizes TAZ to drive gallbladder cancer progression through a feed‐forward transcriptional loop. Structure‐based screening identifies Olaparib as a potent GALNT7 antagonist that disrupts this oncogenic axis, providing an immediate therapeutic strategy for this aggressive malignancy.
Peng Qiu, Ming Zhang, Yunxiang Feng, Yibo Deng, Kai Zhao, Xiangyu Li, Yun Lu, Li Tian, Tao Yang, Wei Yao, Jianming Wang, Zhengdong Deng   +11 more
wiley   +1 more source

Q5® Site-Directed Mutagenesis (E0554) v1

, 2014
This is the protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)

core   +1 more source

HMGCR‐Driven Cholesterol Metabolism Promotes Osteoarthritis Progression by Accelerating Synovial Fibroblast Senescence

Advanced Science, EarlyView.
In the pathological context of osteoarthritis (OA), the phosphorylation of AKT1 at Ser473 enhances its binding to Lys140 of Insig1, which facilitates the formation of AKT1–Insig1 complex. Subsequently, the activation of AKT1 promotes the phosphorylation of Insig1 at Ser189, potentially enhancing the dissociation of Insig1 from sterol regulatory element‑
Xiaoqi Zhang, Siru Zhou, Xi Liu, Wei Xiang, Xiuqin Peng, Yu Tian, Song Li, Hailin Wang, Bo Liao, Shunzheng Fang, Runze Jin, Jinhui Wu, Chuanqing Bai, Wei Zhang, Bo Huang, Bingfei Li, Lifeng Chen, Lin Chen, Bin Zhang, Zhenhong Ni   +19 more
wiley   +1 more source

General Method for Site-Directed Mutagenesis

BioTechniques, 2001
Reiner Andag, Ekkehard Schütz
doaj   +1 more source

Q5® Site-Directed Mutagenesis (E0552) v2

, 2017
This is the protocol for the Q5® Site-Directed Mutagenesis Kit without competent cells
New England Biolabs
core   +1 more source