Results 101 to 110 of about 247,239 (299)
One-Step, Highly Efficient Site-Directed Mutagenesis by Toxic Protein Selection
BioTechniques, 2002 A fast and efficient site-directed mutagenesis method has been developed, using the newly constructed plasmid pTPS19, which expresses the toxic CcdB protein originally encoded by the E. coli F plasmid.
W. Xu +7 more
doaj +1 more source
UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation
Advanced Science, EarlyView.ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro +7 more
wiley +1 more source
Primers used for gene amplification and site-directed mutagenesis. [PDF]
, 2016 Primers used for gene amplification and site-directed mutagenesis.
Caroline Wardrope (3350582) +5 more
core +1 more source
Allosteric modulation of Grb2 drives ligand-dependent signal responses
Biology DirectAdaptor proteins play a crucial role in signal transduction by facilitating the assembly of protein complexes at specific subcellular domains. These multifunctional molecules contain multiple binding modules that enhance the efficiency and flexibility of
Mariana Di Felice +7 more
doaj +1 more source
Auto‐Downregulation of the Florigen FT Production Prevents Precocious Flowering in Plants
Advanced Science, EarlyView.This study shows that the leaf vein‐expressed FD complexes with the florigen FT to bind DNA motifs in FT promoter, which antagonizes CONSTANS‐mediated FT activation to feedback down‐regulate FT expression. This prevents an excessive induction of FT expression by inductive photoperiods, thereby precluding precocious transition to flowering in ...
Shu Tian, Xiao Luo, Bowen Cui, Yuehui He
wiley +1 more source
Oligonucleotides used for the site-directed mutagenesis in this study. [PDF]
, 2015 Oligonucleotides used for the site-directed mutagenesis in this study.
Chan Ding (317445) +6 more
core +1 more source
Citation: 'site-directed mutagenesis' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.12726 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms ...
openaire +1 more source
Advanced Science, EarlyView.The cysteine redox state of the Hendra virus W (WHeV) protein controls assembly into fibrils or amorphous aggregates, with residues 2–29 required for fibrillation. In cells, WHeV forms different types of nuclear condensates. Impaired ability to form redox‐sensitive, non‐filamentous condensates reduces WHeV inhibition of the NF‐κB pathway, while it ...
Frank Gondelaud +12 more
wiley +1 more source
Advanced Science, EarlyView.Mitochondrial SLC25A46 is upregulated in ovarian cancer (OC) and correlates with poor prognosis. SLC25A46 stabilizes CACT to activate fatty acid oxidation, increasing ATP and NADPH production to drive cell proliferation and ferroptosis evasion. SLC25A46 silencing sensitizes OC cells to ferroptosis and carboplatin, offering a promising therapeutic ...
Yunge Gao +9 more
wiley +1 more source
T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis
Biotechnology & Biotechnological Equipment, 2016 Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo +4 more
doaj +1 more source