Results 101 to 110 of about 3,618,606 (252)

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Parameter prediction of site-directed mutagenesis.

, 2015
Parameter prediction of site-directed mutagenesis.
Chun-yu Gao (696324), Chun-li Li (696326), Xian-xian Huang (696323), Qing-jun Zhao (696325)   +3 more
core   +1 more source

From Cell‐Derived Vesicles to Hybrid Nanovectors: Biological Membranes as Functional Blueprints for Gene Delivery

Advanced Healthcare Materials, EarlyView.
Biologically derived and hybrid nonviral nanovectors are examined as distinct but convergent design approaches. Integrating synthetic components with biologically functionalized membranes allows efficient interactions with complex cellular environments.
Clara Baldari, Claudia Leone, Gabriella Leccese, Claudia De Stradis, Alessandra Invidia, Concetta Quintarelli, Biagio De Angelis, Franco Locatelli, Giuseppe Gigli, Gabriele Maiorano, Ilaria Elena Palamà   +10 more
wiley   +1 more source

T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis

Biotechnology & Biotechnological Equipment, 2016
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo, Shuhui Sun, Binghua Xie, Xiao-Jing Zhu, Zhong-Min Dai   +4 more
doaj   +1 more source

Oligonucleotides used for site-directed mutagenesis.

Oligonucleotides used for site-directed mutagenesis.
Shapla Bhattacharya (18388971), Sofija Matisone (12888204), Diana Zelencova-Gopejenko (12888213), Kaspars Tars (1334421), Inara Akopjana (462211), Laura Drunka (9744819), Janis Bogans (667557), Kalvis Brangulis (18388968)   +7 more
core   +1 more source

Deep Contrastive Learning for High‐Throughput Prediction of Drug Resistance Mutations from Sequences

Advanced Science, EarlyView.
This study presents DeepMutDTA, a deep learning framework aimed at predicting mutation‐induced changes in protein‐drug interactions and prioritizing variants potentially linked to drug resistance. Trained on large‐scale data, it incorporates SimSiam‐MuTF, a label‐aware contrastive fine‐tuning strategy that encourages separation between WT and MT ...
Xiaowen Hu, Pan Zhang, Shangqian Wu, Hao Sun, Minwei Li, Sophia Tsoka, Zizhang Sheng, Lei Deng   +7 more
wiley   +1 more source

Allosteric modulation of Grb2 drives ligand-dependent signal responses

Biology Direct
Adaptor proteins play a crucial role in signal transduction by facilitating the assembly of protein complexes at specific subcellular domains. These multifunctional molecules contain multiple binding modules that enhance the efficiency and flexibility of
Mariana Di Felice, Lucrezia Romana Rolfi, Julian Toso, Valeria Pennacchietti, Eduarda S. Ventura, Angelo Toto, Antonella Tramutola, Stefano Gianni   +7 more
doaj   +1 more source

Site Directed Mutagenesis of YDL124w

, 2014
Site Directed Mutagenesis of YDL124w. The purpose of this research project is to better understand the catalytic activity of the Saccharomyces cerevisiae enzyme ...
Owens, Zachary, Smith, Jeremy
core  

Primers for site directed mutagenesis of GGAP2.

, 2013
Primers for site directed mutagenesis of GGAP2.
Chengxi Ren (331176), Michael Ittmann (45547), Jianghua Wang (159271), Yi Cai (233861)   +3 more
core   +1 more source

Rab1A Promotes Hepatic Steatosis by Suppressing Mitophagy via the Raf‐1/ERK1/2/PINK1 Signaling Axis

Advanced Science, EarlyView.
Why does fat accumulate in the liver? Our study reveals Rab1A as the molecular switch that silences the cell's mitochondrial cleanup crew. Turning Rab1A off reactivates mitophagy, clears excess fat, and reverses fatty liver disease in mice—offering a promising new therapeutic target for MASLD, a growing global health challenge.
Li Zhang, Jianhua Li, Huilu Zhang, Qi Qin, Yichen Huang, Xiaofan Tian, Chao Sun, Binbin Li, Zhengxin Wang, Xin Zhang   +9 more
wiley   +1 more source