Results 81 to 90 of about 3,618,606 (252)

Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site

eLife, 2015
Enzymes enable life by accelerating reaction rates to biological timescales. Conventional studies have focused on identifying the residues that have a direct involvement in an enzymatic reaction, but these so-called ‘catalytic residues’ are embedded in ...
Fanny Sunden, Ariana Peck, Julia Salzman, Susanne Ressl, Daniel Herschlag   +4 more
doaj   +1 more source

Program for the site-directed mutagenesis PCR.

, 2018
Program for the site-directed mutagenesis PCR.
Hartmut H. Niemann (5204207), Marcel Frese (4813077), Jörn Kalinowski (23807), Lea Schröder (5204204), Norbert Sewald (542162), Pia R. Neubauer (5204201), Daniel Wibberg (839038), Christiane Widmann (5204198), Tilman Kottke (1273818)   +8 more
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Site-directed mutagenesis studies of conserved and group-specific residues in adenylate-forming enzymes.

, 2018
Site-directed mutagenesis studies of conserved and group-specific residues in adenylate-forming enzymes.
Danielle Leatherby (5714819), Louis Clark (5714816), John Perozich (5714825), Alexander J. Ropelewski (237444), Elizabeth Krilich (5714822)   +4 more
core   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR

Biotechnology & Biotechnological Equipment, 2017
Introducing various mutations at multiple specific sites within a gene requires multiple steps of DNA manipulation, which is the initial, but limiting step of protein structure–function studies.
Fanli Zeng, Yujie Zhang, Ze Zhang, Asrar Ahmad Malik, Yibin Lin   +4 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2.

PLoS ONE, 2015
Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad antimicrobial activities against bacteria and plays an important role in host defense.
Xian-xian Huang, Chun-yu Gao, Qing-jun Zhao, Chun-li Li   +3 more
doaj   +1 more source

Oligonucleotide primers used for site-directed mutagenesis.

, 2017
Oligonucleotide primers used for site-directed mutagenesis.
Sheng Chen (21971), Xuguo Duan (1888171), Jing Wu (54032), Zhu Li (193162)   +3 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source