BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma
FEBS Open Bio, EarlyView.The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru +6 more
wiley +1 more source
A membrane-inserted structural model of the yeast mitofusin Fzo1 [PDF]
, 2017 Mitofusins are large transmembrane GTPases of the dynamin-related protein family, and are required for the tethering and fusion of mitochondrial outer membranes.
A Fiser +78 more
core +4 more sources
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Study on increase of nicotinamide mononucleotide yield by site-directed mutagenesis of nicotinamide phosphoribosyltransferase [PDF]
Zhongguo niangzaoNicotinamide phosphoribosyltransferase (NAMPT) was a key rate-limiting enzyme in the biosynthetic pathway of nicotinamide mononucleotide (NMN). In this study, the NAMPT gene derived from Comamonadaceae bacterium was heterologous expressed in Escherichia ...
DONG Ping, XU Yifan, HOU Ying, LUO Xuegang, ZHANG Lin, QIAO Changsheng
doaj +1 more source
Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension
BioTechniques, 2020 We describe the application of simple cloning by prolonged overlap extension for multiple site-directed mutagenesis in the same plasmid. We show that it is possible to use this technique with very short PCR templates.
Rasmus Hejlesen +1 more
doaj +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
A combined computational-experimental approach to define the structural origin of antibody recognition of sialyl-Tn, a tumor-associated carbohydrate antigen. [PDF]
, 2018 Anti-carbohydrate monoclonal antibodies (mAbs) hold great promise as cancer therapeutics and diagnostics. However, their specificity can be mixed, and detailed characterization is problematic, because antibody-glycan complexes are challenging to ...
Amon, Ron +12 more
core +2 more sources
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source
An efficient one-step site-directed and site-saturation mutagenesis protocol [PDF]
, 2017 We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency.
Baumann, Ulrich +2 more
core
Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide [PDF]
, 2007 Horseradish peroxidase (HRP) is a commonly used enzyme in many biotechnological fields. Improvement of HRP stability would further increase its potential application range.
Arnold +18 more
core +2 more sources