Results 71 to 80 of about 247,239 (299)

Oligonucleotides used for general cloning and site-directed mutagenesis. [PDF]

, 2015
Nucleotides altered by site directed mutagenesis are underlined.Oligonucleotides used for general cloning and site-directed mutagenesis.
Beate Sodeik (191751), Rudolf Bauerfeind (152093), Christina Funk (756280), Verena Raschbichler (756281), Anne Binz (309492), Melanie Ott (26926), Susanne M. Bailer (260258), Claus-Henning Nagel (309485)   +7 more
core   +1 more source

Selection Strategy for Site-Directed Mutagenesis Based on Altered β-Lactamase Specificity

BioTechniques, 1998
Conventional approaches to oligonucleotide-directed mutagenesis rely upon the application of a selection strategy to maximize mutagenesis efficiencies.
Christine A. Andrews, Scott A. Lesley
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Site-Directed Mutagenesis of Green Fluorescent Protein [PDF]

, 2017
Site-Directed Mutagenesis of Green Fluorescent ...
Huynh, Maria
core  

Q5® Site-Directed Mutagenesis (E0554) v1 [PDF]

, 2014
This is the protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)

core   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Q5® Site-Directed Mutagenesis (E0552) v2 [PDF]

, 2017
This is the protocol for the Q5® Site-Directed Mutagenesis Kit without competent cells
New England Biolabs
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Q5® Site-Directed Mutagenesis (E0552) v1 [PDF]

, 2014
This is the protocol for the Q5® Site-Directed Mutagenesis Kit without competent cells

core   +1 more source

Creating Randomized Amino Acid Libraries with the QuikChange® Multi Site-Directed Mutagenesis Kit

BioTechniques, 2002
The QuikChange® Multi Site-Directed Mutagenesis Kit is a simple and efficient method for introducing point mutations at up to five sites simultaneously in plasmid DNA templates. Here we used the QuikChange Multi kit with degenerate (one codon) primers to
Holly H. Hogrefe, Janice Cline, Geri L. Youngblood, Ronda M. Allen   +3 more
doaj   +1 more source