Results 51 to 60 of about 247,239 (299)

Targeting transcription factors associated with hemoglobinopathies: Lessons from successful interventions and implications for cancer

Molecular Oncology, EarlyView.
This review summarizes the transcription factors, repressive chromatin‐modifying complexes, and epigenetic mechanisms that control fetal hemoglobin repression. Notably, many regulators of γ‐globin silencing also function in transcriptional and epigenetic networks that drive cancer, highlighting opportunities to translate advances in hemoglobinopathy ...
Meigen Yu, Puspa Das, Xing Zhang, Xiaodong Cheng   +3 more
wiley   +1 more source

The Primer Generator: A Program that Facilitates the Selection of Oligonucleotides for Site-Directed Mutagenesis

BioTechniques, 1999
Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins.
Alexander Turchin, Joseph F. Lawler
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Oligonucleotide primers used for site-directed mutagenesis. [PDF]

, 2017
Oligonucleotide primers used for site-directed mutagenesis.
Sheng Chen (21971), Xuguo Duan (1888171), Jing Wu (54032), Zhu Li (193162)   +3 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Study on increase of nicotinamide mononucleotide yield by site-directed mutagenesis of nicotinamide phosphoribosyltransferase [PDF]

Zhongguo niangzao
Nicotinamide phosphoribosyltransferase (NAMPT) was a key rate-limiting enzyme in the biosynthetic pathway of nicotinamide mononucleotide (NMN). In this study, the NAMPT gene derived from Comamonadaceae bacterium was heterologous expressed in Escherichia ...
DONG Ping, XU Yifan, HOU Ying, LUO Xuegang, ZHANG Lin, QIAO Changsheng
doaj   +1 more source

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Parameter prediction of site-directed mutagenesis. [PDF]

, 2015
Parameter prediction of site-directed mutagenesis.
Chun-yu Gao (696324), Chun-li Li (696326), Xian-xian Huang (696323), Qing-jun Zhao (696325)   +3 more
core   +1 more source

Identification of a Key Amino Acid of the Human 5-HT2B Serotonin Receptor Important for Sarpogrelate Binding

Journal of Pharmacological Sciences, 2007
Based on radio-ligand binding and molecular modeling studies, sarpogrelate shows a moderate selectivity for 5-HT2B versus 5-HT2A receptors. To confirm the modeling data of sarpogrelate to 5-HT2B receptors predicting interaction of sarpogrelate towards ...
Habib Abul Muntasir, Murad Hossain, Mohiuddin Ahmed Bhuiyan, Tadazumi Komiyama, Takashi Nakamura, Masanobu Ozaki, Takafumi Nagatomo   +6 more
doaj   +1 more source