Results 51 to 60 of about 3,618,606 (252)

Identification and characterization of the functional amino acids at the active center of pig liver thioltransferase by site-directed mutagenesis.

Journal of Biological Chemistry, 1991
By using site-directed mutagenesis techniques, the essential amino acids at the catalytic center of porcine thioltransferase (glutaredoxin) were determined.
Yan-Feng Yang, W. W. Wells
semanticscholar   +1 more source

Primers used for site directed mutagenesis.

, 2015
Primers used for site directed mutagenesis.
Pascual Sanz (208343), Carla Rubio-Villena (761725), Maria Adelaida Garcia-Gimeno (761726)   +2 more
core   +3 more sources

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

Different Approaches For Protein Engineering In Industrial Biotechnology [PDF]

, 2011
Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both.
Muhammad Waheed Akhtar, Israr Khan
core  

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Structural analysis of the FMN binding domain of NADPH-cytochrome P-450 oxidoreductase by site-directed mutagenesis.

Journal of Biological Chemistry, 1989
Comparison of the amino acid sequence of rat liver NADPH-cytochrome P-450 oxidoreductase with that of flavoproteins of known three-dimensional structure suggested that residues Tyr-140 and Tyr-178 are involved in binding of FMN to the protein.
A. Shen, Todd D. Porter, T. E. Wilson, Charles B. Kasper   +3 more
semanticscholar   +1 more source

Primers for site-directed mutagenesis.

, 2014
Note: The mutated sites are underlined in bold and italic.Primers for site-directed mutagenesis.
Haihong Hao (309782), Guyue Cheng (456409), Lingli Huang (309787), Hongmin Ma (625034), Yuanhu Pan (625035), Changcun Liu (625033), Xu Wang (28596), Menghong Dai (309784), Zonghui Yuan (309797)   +8 more
core   +1 more source

Targeting transcription factors associated with hemoglobinopathies: Lessons from successful interventions and implications for cancer

Molecular Oncology, EarlyView.
This review summarizes the transcription factors, repressive chromatin‐modifying complexes, and epigenetic mechanisms that control fetal hemoglobin repression. Notably, many regulators of γ‐globin silencing also function in transcriptional and epigenetic networks that drive cancer, highlighting opportunities to translate advances in hemoglobinopathy ...
Meigen Yu, Puspa Das, Xing Zhang, Xiaodong Cheng   +3 more
wiley   +1 more source

Paclitaxel induces NM2‐dependent cellular contraction through GEF‐H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

Molecular Oncology, EarlyView.
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez, Rafael Pérez‐Díaz, Hugo Ramos‐Solano, Marina Garrido‐Casado, Vanessa C. Talayero, Miguel Vicente‐Manzanares   +5 more
wiley   +1 more source

Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site.

Journal of Biological Chemistry, 1991
The active site of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) has been probed using site-directed mutagenesis and inhibitor binding techniques.
Stephen R. Padgette, Diane B. Ré, C. Gasser, D. Eichholtz, R. Frazier, C. M. Hironaka, E. B. Levine, D. Shah, R. Fraley, G. Kishore   +9 more
semanticscholar   +1 more source