Results 41 to 50 of about 3,618,606 (252)

Identification of amino acid residues in the ligand binding repeats of LDL receptor important for PCSK9 binding[S]

Journal of Lipid Research, 2019
Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDL receptor (LDLR) degradation, increasing plasma levels of LDL cholesterol and the risk of cardiovascular disease.
Shi-jun Deng, Adekunle Alabi, Hong-mei Gu, Ayinuer Adijiang, Shucun Qin, Da-wei Zhang   +5 more
doaj   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

The Primer Generator: A Program that Facilitates the Selection of Oligonucleotides for Site-Directed Mutagenesis

BioTechniques, 1999
Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins.
Alexander Turchin, Joseph F. Lawler
doaj   +1 more source

site‐directed mutagenesis

, 2012
In vivo site-directed mutagenesis by ssDNA recombineering is a facile method to change the color of fluorescent proteins without cloning. Two different starting alleles of GFP were targeted for mutagenesis: gfpmut3* residing in the E.
Ceballos, Melvys Valledor, Richard S. Myers, Hu, Qinghua, Schiller, Paul, Myers, Richard S, Melvys Valledor, Paul Schiller, Qinghua Hu   +7 more
core   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Discrimination between oxygen and carbon monoxide and inhibition of autooxidation by myoglobin. Site-directed mutagenesis of the distal histidine.

Journal of Biological Chemistry, 1989
Sperm whale myoglobin mutants were constructed using site-directed mutagenesis to replace the highly conserved distal histidine residue (His(E7)-64).
B. Springer, Karen D. Egeberg, Stephen G. Sligar, R. J. Rohlfs, A. J. Mathews, J. S. Olson   +5 more
semanticscholar   +1 more source

Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity

Biochemistry and Biophysics Reports, 2016
Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves, Joana Lamego, Tiago Bandeiras, Rute Castro, Hélio Tomás, Ana Sofia Coroadinha, Júlia Costa, Ana Luisa Simplício   +7 more
doaj   +1 more source

Directed evolution of transketolase, a carbon-carbon forming enzyme. [PDF]

, 2005
The enzyme transketolase (Enzyme Commission number: 2.2.1.1) has significant potential as a biocatalyst in the production of pharmaceuticals and fine chemicals.
Miller, O.J.
core  

Mutagenesis sites, primers and templates for site-directed mutagenesis.

, 2013
Mutagenesis sites, primers and templates for site-directed mutagenesis.
James M. Mason (103110), Robert M. Petrovich (460283), Sukhdev S. Brar (460282), Jason G. Williams (366459)   +3 more
core   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source