Results 31 to 40 of about 3,618,606 (252)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

High-throughput site-directed mutagenesis using oligonucleotides synthesized on DNA chips

BioTechniques, 2005
Site-directed mutagenesis has greatly helped researchers both to understand the precise role of specific residues in coding sequences and to generate variants of proteins that have acquired new characteristics.
Didier Saboulard, Vincent Dugas, Mehdi Jaber, Jérôme Broutin, Eliane Souteyrand, Julien Sylvestre, Marc Delcourt   +6 more
doaj   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

Journal of Biological Chemistry, 1985
Site-directed mutagenesis can be employed to alter activity critical residues in proteins which are susceptible to chemical oxidation. Previous studies have implicated methionine 222 as a primary site for oxidative inactivation of subtilisin (Stauffer, C.
D. Estell, Thomas P. Graycar, James A. Wells   +2 more
semanticscholar   +1 more source

Involvement of BcYak1 in the Regulation of Vegetative Differentiation and Adaptation to Oxidative Stress of Botrytis cinerea

Frontiers in Microbiology, 2018
Yak1, a member of the dual-specificity tyrosine phosphorylation-regulated protein kinases, plays an important role in diverse cellular processes in fungi. However, to date, the role of BcYak1 in Botrytis cinerea, the causal agent of gray mold diseases in
Qianqian Yang, Jianan Zhang, Jicheng Hu, Xue Wang, Binna Lv, Wenxing Liang   +5 more
doaj   +1 more source

Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve

Frontiers in Microbiology, 2021
Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt, Emily C. Hoedt, Francesca Bottacini, Francesca Bottacini, Nora Cash, Roger S. Bongers, Kees van Limpt, Kaouther Ben Amor, Jan Knol, Jan Knol, John MacSharry, John MacSharry, John MacSharry, Douwe van Sinderen, Douwe van Sinderen   +14 more
doaj   +1 more source

Primers of site-directed mutagenesis.

, 2014
Primers of site-directed mutagenesis.
Cheng Liu (83733), Wei Zhang (405), Yue Han (560788), Xi Chen (35903)   +3 more
core   +1 more source

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Primers used for site-directed mutagenesis.

, 2016
Primers used for site-directed mutagenesis.
Xuguo Duan (1888171), Jing Wu (54032), Yixin Ai (1888174), Sheng Cheng (202385)   +3 more
core   +2 more sources